Spatial organization of calcium dynamics in growth cones of sensory neurones.

The concentration of calcium ions in the cytosol ([Ca2+]i) has a dominant influence on neuronal development. A [Ca2+]i rise can, depending on the amplitude and location, promote outgrowth or dramatically inhibit it. We have used the fluorescent calcium indicators Fura-2 and Fura-2 dextran to measure [Ca2+]i dynamics in sensory neurones from the adult rat. [Ca2+]i was low and uniform in advancing growth cones, even during specific behaviours such as protrusion, filling and consolidation. A brief train of action potentials caused [Ca2+]i to rise at the extreme leading edge of the growth cone. [Ca2+]i changes in more proximal regions of the growth cone were much smaller. This spatially organized [Ca2+]i change, which may result from a concentration of calcium channels at the growth cone leading edge, is likely to function in spontaneously active regenerating axons in vivo to specifically activate calcium-dependent processes at the growth cone tip.