Literature DB >> 885562

Organ culture of human articular cartilage: studies on sulphated glycosaminoglycan synthesis.

L S McKenzie, B A Horsburgh, P Ghosh, T K Taylor.   

Abstract

An organ culture system is described for adult human articular cartilage obtained from joints after femoral head replacement operations. Cartilage slices maintain maximal viability for 2 days in culture as assessed by uptake of [3H]uridine and [3H]leucine into whole tissue, and 35SO4 into sulphated glycosaminoglycans (GAGs). Since GAGs are the components of cartilage matrix, the depletion of which is associated with osteoarthrosis, a method for measuring sulphated GAG synthesis in culture has been investigated.

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Year:  1977        PMID: 885562     DOI: 10.1007/bf02615102

Source DB:  PubMed          Journal:  In Vitro        ISSN: 0073-5655


  20 in total

1.  A modified uronic acid carbazole reaction.

Authors:  T BITTER; H M MUIR
Journal:  Anal Biochem       Date:  1962-10       Impact factor: 3.365

2.  The culture of mature organs in a synthetic medium.

Authors:  O A TROWELL
Journal:  Exp Cell Res       Date:  1959-01       Impact factor: 3.905

3.  The long-term maintenance of tumor explants.

Authors:  F C JENSEN; G A CASTELLANO
Journal:  Cancer Chemother Rep       Date:  1960-07

4.  The uptake of labelled sulphate by human cartilage cells and its use as a test for viability.

Authors:  R C CURRAN; T GIBSON
Journal:  Proc R Soc Lond B Biol Sci       Date:  1956-03-13

Review 5.  Glycosaminoglycans. A biochemical and clinical review.

Authors:  S I Lamberg; A C Stoolmiller
Journal:  J Invest Dermatol       Date:  1974-12       Impact factor: 8.551

6.  Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips.

Authors:  H J Mankin; L Lippiello
Journal:  J Bone Joint Surg Am       Date:  1970-04       Impact factor: 5.284

7.  Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data.

Authors:  H J Mankin; H Dorfman; L Lippiello; A Zarins
Journal:  J Bone Joint Surg Am       Date:  1971-04       Impact factor: 5.284

8.  Calf anticular cartilage in organ culture in a chemically defined medium. I. Autoradiographic study after [35S]sulfate labeling.

Authors:  A Bergenholtz; R K Lemperg
Journal:  In Vitro       Date:  1975 Sep-Oct

9.  Glycosaminoglycan turn-over in articular cartilage.

Authors:  A Maroudas
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1975-07-17       Impact factor: 6.237

10.  Effect of anti-inflammatory drugs on sulphated glycosaminoglycan synthesis in aged human articular cartilage.

Authors:  L S McKenzie; B A Horsburgh; P Ghosh; T K Taylor
Journal:  Ann Rheum Dis       Date:  1976-12       Impact factor: 19.103

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  1 in total

1.  Metabolic responses of cartilage in experimentally induced osteoarthritis.

Authors:  R W Moskowitz; V M Goldberg; C J Malemud
Journal:  Ann Rheum Dis       Date:  1981-12       Impact factor: 19.103

  1 in total

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