An improved method for the detection of peptide-induced upregulation of HLA-A2 molecules on TAP-deficient T2 cells.

Peptide-induced upregulation of MHC class I on TAP-deficient T2 cells is used to monitor peptide binding to class I molecules. We present a temperature-dependent modification of the T2 assay which increases the sensitivity of peptide binding to MHC class I with several orders of magnitude.