BACKGROUND: Cytotoxic T cell (CTL) responses may influence the course of a hepatitis C infection. CTL recognize viral peptides on HLA class I molecules. Antigenic peptides for class I presentation are generated by proteasomes and transported into the endoplasmic reticulum by the TAP proteins. It is unknown whether polymorphisms of the antigen-processing genes might influence the development of chronic hepatitis C. METHODS: 75 patients with chronic hepatitis C and 99 unrelated controls were typed for TAP and LMP2 polymorphisms. Typing was done by allele-specific PCR techniques and PCR with subsequent single-stranded confirmation polymorphism (SSCP) analysis. RESULTS: Apart from minor increases of the TAP2*0101 allele and LMP2 heterozygotes in the chronic hepatitis group there were no statistically significant differences between the two groups. CONCLUSIONS: These findings suggest, that disease association for chronic hepatitis C does not extend beyond the HLA-DQ locus. Although TAP and LMP play an important role in the processing of viral antigens, their limited polymorphisms do not show any association with the development of chronic hepatitis C.
BACKGROUND:Cytotoxic T cell (CTL) responses may influence the course of a hepatitis C infection. CTL recognize viral peptides on HLA class I molecules. Antigenic peptides for class I presentation are generated by proteasomes and transported into the endoplasmic reticulum by the TAP proteins. It is unknown whether polymorphisms of the antigen-processing genes might influence the development of chronic hepatitis C. METHODS: 75 patients with chronic hepatitis C and 99 unrelated controls were typed for TAP and LMP2 polymorphisms. Typing was done by allele-specific PCR techniques and PCR with subsequent single-stranded confirmation polymorphism (SSCP) analysis. RESULTS: Apart from minor increases of the TAP2*0101 allele and LMP2 heterozygotes in the chronic hepatitis group there were no statistically significant differences between the two groups. CONCLUSIONS: These findings suggest, that disease association for chronic hepatitis C does not extend beyond the HLA-DQ locus. Although TAP and LMP play an important role in the processing of viral antigens, their limited polymorphisms do not show any association with the development of chronic hepatitis C.
Authors: H L Tillmann; D F Chen; C Trautwein; V Kliem; A Grundey; A Berning-Haag; K Böker; S Kubicka; L Pastucha; W Stangel; M P Manns Journal: Gut Date: 2001-05 Impact factor: 23.059