Literature DB >> 8853012

Reversed-phase HPLC separation of proteins on chemically bonded silica gel columns.

N Nimura1, H Itoh.   

Abstract

Reversed-phase high-performance liquid chromatographic (RP-HPLC) separation of proteins on chemically bonded silica gel columns is described. Efficiency of nonporous alkylsilyl bonded silica gel is compared with that of a macroporous gel that has been widely used for the purpose. A comparative study of the separation under conventional and fast separation conditions is also given. The fast separation technique on the nonporous reversed-phase column has the advantage of improving the recovery of late-eluting hydrophobic and large proteins, such as ovalbumin and apoferritin.

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Year:  1996        PMID： 8853012     DOI： 10.1007/BF02762408

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  8 in total

1.  Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. III. Retention and selectivity of proteins and peptides in gradient elution on non-porous monodisperse 1.5-microns reversed-phase silicas.

Authors:  G Jilge; R Janzen; H Giesche; K K Unger; J N Kinkel; M T Hearn
Journal:  J Chromatogr       Date:  1987-06-26

2.  Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. IV. Mobile phase and surface-mediated effects on recovery of native proteins in gradient elution on non-porous, monodisperse 1.5-microns reversed-phase silicas.

Authors:  R Janzen; K K Unger; H Giesche; J N Kinkel; M T Hearn
Journal:  J Chromatogr       Date:  1987-06-26

3.  Fast protein separation by reversed-phase high-performance liquid chromatography on octadecylsilyl-bonded nonporous silica gel. II. Improvement in recovery of hydrophobic proteins.

Authors:  H Itoh; N Nimura; T Kinoshita; N Nagae; M Nomura
Journal:  Anal Biochem       Date:  1991-11-15       Impact factor: 3.365

4.  Protein separations on reversed-phase high-performance liquid chromatography minicolumns.

Authors:  R M Moore; R R Walters
Journal:  J Chromatogr       Date:  1984-12-28

Review 5.  High-performance liquid chromatography of biopolymers.

Authors:  F E Regnier
Journal:  Science       Date:  1983-10-21       Impact factor: 47.728

6.  The importance of silica type for reverse-phase protein separations.

Authors:  J D Pearson; N T Lin; F E Regnier
Journal:  Anal Biochem       Date:  1982-07-15       Impact factor: 3.365

7.  Retention model for proteins in reversed-phase liquid chromatography.

Authors:  X Geng; F E Regnier
Journal:  J Chromatogr       Date:  1984-07-27

8.  Gradient LC separation of macromolecules: theory and mechanism.

Authors:  D W Armstrong; R E Boehm
Journal:  J Chromatogr Sci       Date:  1984-09       Impact factor: 1.618
  8 in total

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