M C Schiewe1. 1. Institute for Fertility Research, California Fertility Associates, Santa Monica 90404, USA.
Abstract
PURPOSE: Our purpose was to use effectively a simplified approach for intracytoplasmic sperm injection (ICSI) that achieves high levels of fertilization, embryo cleavage, and subsequent establishment of clinical pregnancies. METHODS: In a multicenter clinical analysis, 1814 mature oocytes were injected with a single sperm using a finely beveled (30 degrees) pipette without a spiked tip that was backfilled with an inert, silicone polymer. The injection system was composed of a sterile water-filled tubing interconnecting the pipette and a 20-gauge needle attached to a three-way stopcock and 5-ml disposable syringe. The efficiency of the ICSI procedure was assessed by analyzing fertilization, lysis, and cleavage rates, as well as pregnancy outcomes. RESULTS: High levels of normal fertilization (2PN; 56.4-73.5% of all eggs injected) and reasonably low lysis rates (9-16%) were produced. The efficiency of embryo formation was high (87-93%), with only 6 of 177 (3.4%) patients failing to produce an embryo. Clinical pregnancies were established by each IVF center (18-34% ongoing pregnancy/birth rate). CONCLUSIONS: Effective ICSI was achieved using a novel, simplified technique involving sharply beveled pipettes (without additional tip modification), simple sperm washing/handling procedures, and a disposable syringe injection system that offered extremely fine control for sperm manipulation.
PURPOSE: Our purpose was to use effectively a simplified approach for intracytoplasmic sperm injection (ICSI) that achieves high levels of fertilization, embryo cleavage, and subsequent establishment of clinical pregnancies. METHODS: In a multicenter clinical analysis, 1814 mature oocytes were injected with a single sperm using a finely beveled (30 degrees) pipette without a spiked tip that was backfilled with an inert, silicone polymer. The injection system was composed of a sterile water-filled tubing interconnecting the pipette and a 20-gauge needle attached to a three-way stopcock and 5-ml disposable syringe. The efficiency of the ICSI procedure was assessed by analyzing fertilization, lysis, and cleavage rates, as well as pregnancy outcomes. RESULTS: High levels of normal fertilization (2PN; 56.4-73.5% of all eggs injected) and reasonably low lysis rates (9-16%) were produced. The efficiency of embryo formation was high (87-93%), with only 6 of 177 (3.4%) patients failing to produce an embryo. Clinical pregnancies were established by each IVF center (18-34% ongoing pregnancy/birth rate). CONCLUSIONS: Effective ICSI was achieved using a novel, simplified technique involving sharply beveled pipettes (without additional tip modification), simple sperm washing/handling procedures, and a disposable syringe injection system that offered extremely fine control for sperm manipulation.
Authors: R J Sherins; L P Thorsell; A Dorfmann; L Dennison-Lagos; L P Calvo; L Krysa; C B Coulam; J D Schulman Journal: Fertil Steril Date: 1995-08 Impact factor: 7.329