| Literature DB >> 8852725 |
T Page1, C Sherwood, J D Connor, T Tarnowski.
Abstract
A fast, simple, and cost-effective HPLC method for the quantitation of the antiviral drug ganciclovir is described. The serum samples are extracted with perchloric acid and neutralized with potassium phosphate buffer, and urine samples are diluted with distilled water. A reversed-phase column with isocratic elution by 15 mM potassium phosphate buffer (pH 2.5) containing 0.25% acetonitrile is used to separate ganciclovir; quantitation is by UV absorbance at 254 nm. Total turnaround time is 22 min; more than 3000 samples can be run on a single column without loss of peak quality. The limit of quantitation is 0.05 micrograms/ml. Recoveries varied from 91 to 107% with coefficients of variation ranging from 0.387 to 7.95%.Entities:
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Year: 1996 PMID： 8852725 DOI： 10.1016/0378-4347(95)00381-9
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495