Literature DB >> 8851562

Location of the alpha-amylase gene in rumen Streptococcus bovis strains distinguished by unstable amylase activity.

M Mareková1, Z Jonecová, V Kmeĭ.   

Abstract

Genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminal Streptococcus bovis strains was investigated. Two strains Amy+ and Amy- were obtained. Loss of amylase activity connected with the loss of plasmid DNA was not found in these strains. The presence of the gene responsible for the amylase activity in the chromosome of these strains was revealed by hybridization of the alpha-amylase gene on pJK108 against chromosomal DNA of S. bovis and Bacillus subtilis after a complete restriction with EcoRI.

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Year:  1995        PMID: 8851562     DOI: 10.1007/bf02815419

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN: 0015-5632            Impact factor:   2.099


  11 in total

1.  Genetic analysis of the promoter region of the Bacillus subtilis alpha-amylase gene.

Authors:  M J Weickert; G H Chambliss
Journal:  J Bacteriol       Date:  1989-07       Impact factor: 3.490

2.  Occurrence of conjugative amylolytic activity in rumen lactobacilli.

Authors:  V Kmet; P Javorský; R Nemcová; J Kopecný; K Boda
Journal:  Zentralbl Mikrobiol       Date:  1989

3.  Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.

Authors:  M J Weickert; G H Chambliss
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

Review 4.  Extracellular enzyme synthesis in the genus Bacillus.

Authors:  F G Priest
Journal:  Bacteriol Rev       Date:  1977-09

5.  A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.

Authors:  G Dretzen; M Bellard; P Sassone-Corsi; P Chambon
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

6.  Development of a DNA probe for Streptococcus bovis by using a cloned amylase gene.

Authors:  T R Whitehead; M A Cotta
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

7.  Catabolite repression of alpha-amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacl and galR repressors.

Authors:  T M Henkin; F J Grundy; W L Nicholson; G H Chambliss
Journal:  Mol Microbiol       Date:  1991-03       Impact factor: 3.501

8.  Molecular cloning and expression of two alpha-amylase genes from Streptococcus bovis 148 in Escherichia coli.

Authors:  E Satoh; Y Niimura; T Uchimura; M Kozaki; K Komagata
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

9.  Regulation and cloning of the gene encoding amylase activity of the ruminal bacterium Streptococcus bovis.

Authors:  M A Cotta; T R Whitehead
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792

10.  Compositions and characteristics of strains of Streptococcus bovis.

Authors:  J B Russell; P H Robinson
Journal:  J Dairy Sci       Date:  1984-07       Impact factor: 4.034

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