Literature DB >> 8850552

Porcine fibrinogen glycopeptides: substrates for detecting endo-beta-N-acetylglucosaminidases F2 and F3(1).

T H Plummer1, A W Phelan, A L Tarentino.   

Abstract

Two different glycopeptides were isolated in high yield from a thermolytic digest of porcine fibrinogen. Edman analysis established their sequences as Val-Glu-Asn(CHO)-Lys and Val-Gly-Glu-Asn(CHO)-Arg. These sequences are nearly identical to the two human fibrinogen glycopeptides, Val-Glu-Asn(CHO)-Lys (gamma-chain), and Met-Gly-Glu-Asn(CHO)-Arg (beta-chain). The predominant carbohydrate moiety of both asialoglycopeptides was a biantennary oligosaccharide with a core alpha(1-->6)-linked fucose as reported earlier (Da Silva et al. (1994) Arch. Biochem. Biophys. 312, 151-157). Both glycopeptides can be dansylated and used as sensitive substrates for Flavobacterium meningosepticum endo-beta-N-acetylglucosaminidases F2 and F3. Porcine fibrinogen represents the best source for substrates with this oligosaccharide type that can be reliably produced in multimicromole quantities.

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Year:  1996        PMID： 8850552     DOI： 10.1006/abio.1996.0096

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Unusual transglycosylation activity of Flavobacterium meningosepticum endoglycosidases enables convergent chemoenzymatic synthesis of core fucosylated complex N-glycopeptides.

Authors:  Wei Huang; Jie Li; Lai-Xi Wang
Journal:  Chembiochem       Date:  2011-03-04       Impact factor: 3.164

2.  Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM.

Authors:  Junfeng Ma; Miloslav Sanda; Renhuizi Wei; Lihua Zhang; Radoslav Goldman
Journal:  J Proteomics       Date:  2018-02-07       Impact factor: 4.044

3.  Endo-F3 Glycosynthase Mutants Enable Chemoenzymatic Synthesis of Core-fucosylated Triantennary Complex Type Glycopeptides and Glycoproteins.

Authors:  John P Giddens; Joseph V Lomino; Mohammed N Amin; Lai-Xi Wang
Journal:  J Biol Chem       Date:  2016-03-10       Impact factor: 5.157

Review 4.  Applications and continued evolution of glycan imaging mass spectrometry.

Authors:  Colin T McDowell; Xiaowei Lu; Anand S Mehta; Peggi M Angel; Richard R Drake
Journal:  Mass Spectrom Rev       Date:  2021-08-15       Impact factor: 10.946

Review 5.  Sculpting therapeutic monoclonal antibody N-glycans using endoglycosidases.

Authors:  Beatriz Trastoy; Jonathan J Du; Mikel García-Alija; Chao Li; Erik H Klontz; Lai-Xi Wang; Eric J Sundberg; Marcelo E Guerin
Journal:  Curr Opin Struct Biol       Date:  2022-01-05       Impact factor: 6.809

6.  Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.

Authors:  Haruka Seki; Yibo Huang; Takatoshi Arakawa; Chihaya Yamada; Takashi Kinoshita; Shogo Iwamoto; Yujiro Higuchi; Kaoru Takegawa; Shinya Fushinobu
Journal:  J Biol Chem       Date:  2019-09-23       Impact factor: 5.157

7.  Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.

Authors:  Feng Tang; Mang Zhou; Ken Qin; Wei Shi; Ansor Yashinov; Yang Yang; Liyun Yang; Dongliang Guan; Lei Zhao; Yubo Tang; Yujie Chang; Lifen Zhao; Huaiyu Yang; Hu Zhou; Ruimin Huang; Wei Huang
Journal:  Nat Chem Biol       Date:  2020-06-01       Impact factor: 15.040

8.  New Enzymatic Approach to Distinguish Fucosylation Isomers of N-Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry.

Authors:  Connor A West; Hongyan Liang; Richard R Drake; Anand S Mehta
Journal:  J Proteome Res       Date:  2020-06-08       Impact factor: 4.466

9.  General and Robust Chemoenzymatic Method for Glycan-Mediated Site-Specific Labeling and Conjugation of Antibodies: Facile Synthesis of Homogeneous Antibody-Drug Conjugates.

Authors:  Xiao Zhang; Chong Ou; Huiying Liu; Sunaina Kiran Prabhu; Chao Li; Qiang Yang; Lai-Xi Wang
Journal:  ACS Chem Biol       Date:  2021-09-27       Impact factor: 5.100

10.  Chemoenzymatic glycan-selective remodeling of a therapeutic lysosomal enzyme with high-affinity M6P-glycan ligands. Enzyme substrate specificity is the name of the game.

Authors:  Xiao Zhang; Huiying Liu; Naresh Meena; Chao Li; Guanghui Zong; Nina Raben; Rosa Puertollano; Lai-Xi Wang
Journal:  Chem Sci       Date:  2021-08-19       Impact factor: 9.825
  10 in total

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