Literature DB >> 8849218

Comparative evaluation of microplate enzyme-linked immunosorbent assay versus plaque reduction assay for antiviral susceptibility testing of herpes simplex virus isolates.

S Safrin1, E Palacios, B J Leahy.   

Abstract

We tested the antiviral susceptibilities of 30 clinical isolates of herpes simplex virus using the microplate in situ enzyme-linked immunosorbent assay (MISE) and the plaque reduction assay (PRA). There was concordance for 26 of 30 acyclovir results and all 30 foscarnet results. MISE and PRA results each predicted the response to acyclovir in 12 of 14 instances and the response to foscarnet in 8 instances. MISE is more rapid than PRA, has an objective endpoint, and correlates well with the clinical response to therapy.

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Year:  1996        PMID: 8849218      PMCID: PMC163250     

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN: 0066-4804            Impact factor:   5.191


  9 in total

1.  A comparative evaluation of three methods of antiviral susceptibility testing of clinical herpes simplex virus isolates.

Authors:  S Safrin; L Phan; T Elbeik
Journal:  Clin Diagn Virol       Date:  1995-07

2.  Improved DNA hybridization method for detection of acyclovir-resistant herpes simplex virus.

Authors:  E M Swierkosz; D R Scholl; J L Brown; J D Jollick; C A Gleaves
Journal:  Antimicrob Agents Chemother       Date:  1987-10       Impact factor: 5.191

3.  Antiviral susceptibility testing with a cell line which expresses beta-galactosidase after infection with herpes simplex virus.

Authors:  P Tebas; E C Stabell; P D Olivo
Journal:  Antimicrob Agents Chemother       Date:  1995-06       Impact factor: 5.191

4.  Correlation between response to acyclovir and foscarnet therapy and in vitro susceptibility result for isolates of herpes simplex virus from human immunodeficiency virus-infected patients.

Authors:  S Safrin; T Elbeik; L Phan; D Robinson; J Rush; A Elbaggari; J Mills
Journal:  Antimicrob Agents Chemother       Date:  1994-06       Impact factor: 5.191

5.  Standardisation of a microplate in situ ELISA (MISE-test) for the susceptibility testing of herpes simplex virus to acyclovir.

Authors:  B J Leahy; K J Christiansen; G Shellam
Journal:  J Virol Methods       Date:  1994-06       Impact factor: 2.014

6.  A colorimetric assay for the measurement of the sensitivity of herpes simplex viruses to antiviral agents.

Authors:  C McLaren; M N Ellis; G A Hunter
Journal:  Antiviral Res       Date:  1983-11       Impact factor: 5.970

7.  Plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates.

Authors:  J L Martin; M N Ellis; P M Keller; K K Biron; S N Lehrman; D W Barry; P A Furman
Journal:  Antimicrob Agents Chemother       Date:  1985-08       Impact factor: 5.191

8.  Foscarnet-resistant herpes simplex virus infection in patients with AIDS.

Authors:  S Safrin; S Kemmerly; B Plotkin; T Smith; N Weissbach; D De Veranez; L D Phan; D Cohn
Journal:  J Infect Dis       Date:  1994-01       Impact factor: 5.226

9.  Herpes simplex virus variants restraint to high concentrations of acyclovir exist in clinical isolates.

Authors:  D S Parris; J E Harrington
Journal:  Antimicrob Agents Chemother       Date:  1982-07       Impact factor: 5.191

  9 in total
  4 in total

Review 1.  Resistance of herpes simplex viruses to nucleoside analogues: mechanisms, prevalence, and management.

Authors:  Jocelyne Piret; Guy Boivin
Journal:  Antimicrob Agents Chemother       Date:  2010-11-15       Impact factor: 5.191

2.  Application of real-time PCR for determination of antiviral drug susceptibility of herpes simplex virus.

Authors:  Růzena Stránská; Anton M van Loon; Merjo Polman; Rob Schuurman
Journal:  Antimicrob Agents Chemother       Date:  2002-09       Impact factor: 5.191

3.  Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity.

Authors:  Carlos Nozawa; Lilian Yumi Hattori; Ligia Carla Faccin Galhardi; Nayara Lopes; Wesley Andrade Bomfim; Ligyana Korki de Cândido; Elbens Marcos Minoreli de Azevedo; Airton dos Santos Gon; Rosa Elisa Carvalho Linhares
Journal:  An Bras Dermatol       Date:  2014 May-Jun       Impact factor: 1.896

Review 4.  Laboratory diagnosis of viral infection.

Authors:  David R Peaper; Marie Louise Landry
Journal:  Handb Clin Neurol       Date:  2014
  4 in total

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