Improving the specificity of indirect immunofluorescence for the serological diagnosis of bovine anaplasmosis.

Fluorescing inclusion appendages were detected consistently in preparations of Anaplasma centrale and Anaplasma marginale when they were used as antigen in indirect immunofluorescence serological tests for the diagnosis of anaplasmosis in cattle. The presence of the inclusion appendages made it possible to confirm the specificity of the immunofluorescent reaction and to determine end-points with accuracy.