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Literature DB >> 88460

Simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions.

Abstract

A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle.

Entities: Chemical

Mesh：

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Year: 1979 PMID： 88460 PMCID： PMC273043 DOI： 10.1128/jcm.9.3.437-440.1979

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

6 in total

1. Method for counting Rickettsiae and Chlamydiae in purified suspensions.

Authors: R Silberman; P Fiset
Journal: J Bacteriol Date: 1968-01 Impact factor: 3.490

2. Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation.

Authors: E Weiss; J C Coolbaugh; J C Williams
Journal: Appl Microbiol Date: 1975-09

3. In vitro studies on rickettsia-host cell interactions: intracellular growth cycle of virulent and attenuated Rickettsia prowazeki in chicken embryo cells in slide chamber cultures.

Authors: C L Wisseman; A D Waddell
Journal: Infect Immun Date: 1975-06 Impact factor: 3.441

4. Limits of rickettsial infectivity.

Authors: R Ormsbee; M Peacock; R Gerloff; G Tallent; D Wike
Journal: Infect Immun Date: 1978-01 Impact factor: 3.441

5. Effects of various suspending media on plaque formation by rickettsiae in tissue culture.

Authors: D A Wike; R A Ormsbee; G Tallent; M G Peacock
Journal: Infect Immun Date: 1972-10 Impact factor: 3.441

6. In vitro studies on Rickettsia-host cell interactions: lag phase in intracellular growth cycle as a function of stage of growth of infecting Rickettsia prowazeki, with preliminary observations on inhibition of rickettsial uptake by host cell fragments.

Authors: C L Wisseman; A D Waddell; D J Silverman
Journal: Infect Immun Date: 1976-06 Impact factor: 3.441

6 in total

12 in total

1. Evaluation of a PCR assay for quantitation of Rickettsia rickettsii and closely related spotted fever group rickettsiae.

Authors: Marina E Eremeeva; Gregory A Dasch; David J Silverman
Journal: J Clin Microbiol Date: 2003-12 Impact factor: 5.948

Simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions.

1. Method for counting Rickettsiae and Chlamydiae in purified suspensions.

2. Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation.

3. In vitro studies on rickettsia-host cell interactions: intracellular growth cycle of virulent and attenuated Rickettsia prowazeki in chicken embryo cells in slide chamber cultures.

4. Limits of rickettsial infectivity.

5. Effects of various suspending media on plaque formation by rickettsiae in tissue culture.

6. In vitro studies on Rickettsia-host cell interactions: lag phase in intracellular growth cycle as a function of stage of growth of infecting Rickettsia prowazeki, with preliminary observations on inhibition of rickettsial uptake by host cell fragments.

1. Evaluation of a PCR assay for quantitation of Rickettsia rickettsii and closely related spotted fever group rickettsiae.

2. Protein synthesis by intact Coxiella burnetii cells.

Review 3. Q fever and Coxiella burnetii: a model for host-parasite interactions.

4. Characteristics of Rickettsia mooseri infection of normal and immune mice.

5. Some characteristics of heavy and light bands of Rickettsia prowazekii on Renografin gradients.

6. Role of antibody in Coxiella burnetii infection.

7. Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components.

8. Role of macrophages in innate and acquired host resistance to experimental scrub typhus infection of inbred mice.

9. Rickettsia rickettsii-induced cellular injury of human vascular endothelium in vitro.

10. Effect of immune serum on infectivity of Rickettsia tsutsugamushi.