Literature DB >> 8845771

High level expression and crystallization of recombinant human cathepsin S.

D Brömme1, M E McGrath.   

Abstract

We have expressed active human cathepsin S to 60 mg/L in Sf9 cells using a baculovirus system. Production of milligram quantities has facilitated crystallographic studies to determine the structure of this enzyme, which has unique properties among lysosomal cysteine proteinases. Recombinant, irreversibly inhibited cathepsin S was crystallized from ammonium phosphate at 17 degrees C. The crystals diffract to at least 2.3 A, and belong to the orthorhombic crystal system with a primitive lattice. Approximate cell dimensions are: a = 37.7 A, b = 73.9 A, and c = 106.7 A. There is most likely one molecule per asymmetric unit.

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Year:  1996        PMID： 8845771      PMCID： PMC2143400          DOI： 10.1002/pro.5560050426

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  10 in total

1.  Sequence analysis, tissue distribution, and expression of rat cathepsin S.

Authors:  S Petanceska; L Devi
Journal:  J Biol Chem       Date:  1992-12-25       Impact factor: 5.157

2.  Phylogenetic conservation of cysteine proteinases. Cloning and expression of a cDNA coding for human cathepsin S.

Authors:  B Wiederanders; D Brömme; H Kirschke; K von Figura; B Schmidt; C Peters
Journal:  J Biol Chem       Date:  1992-07-05       Impact factor: 5.157

3.  Acid sulphydryl protease from calf lymph nodes.

Authors:  T Turnsek; I Kregar; D Lebez
Journal:  Biochim Biophys Acta       Date:  1975-10-22

4.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

5.  Vinyl sulfones as mechanism-based cysteine protease inhibitors.

Authors:  J T Palmer; D Rasnick; J L Klaus; D Brömme
Journal:  J Med Chem       Date:  1995-08-18       Impact factor: 7.446

6.  The specificity of bovine spleen cathepsin S. A comparison with rat liver cathepsins L and B.

Authors:  D Brömme; A Steinert; S Friebe; S Fittkau; B Wiederanders; H Kirschke
Journal:  Biochem J       Date:  1989-12-01       Impact factor: 3.857

7.  Functional expression of human cathepsin S in Saccharomyces cerevisiae. Purification and characterization of the recombinant enzyme.

Authors:  D Brömme; P R Bonneau; P Lachance; B Wiederanders; H Kirschke; C Peters; D Y Thomas; A C Storer; T Vernet
Journal:  J Biol Chem       Date:  1993-03-05       Impact factor: 5.157

8.  Cathepsin S. The cysteine proteinase from bovine lymphoid tissue is distinct from cathepsin L (EC 3.4.22.15).

Authors:  H Kirschke; I Schmidt; B Wiederanders
Journal:  Biochem J       Date:  1986-12-01       Impact factor: 3.857

9.  Cathepsin S from bovine spleen. Purification, distribution, intracellular localization and action on proteins.

Authors:  H Kirschke; B Wiederanders; D Brömme; A Rinne
Journal:  Biochem J       Date:  1989-12-01       Impact factor: 3.857

10.  Differential distribution of messenger RNAs for cathepsins B, L and S in adult rat brain: an in situ hybridization study.

Authors:  S Petanceska; S Burke; S J Watson; L Devi
Journal:  Neuroscience       Date:  1994-04       Impact factor: 3.590
  10 in total
  6 in total

1.  Purification and characterization of a new cystatin inhibitor from Taiwan cobra (Naja naja atra) venom.

Authors:  M Brillard-Bourdet; V Nguyên; M Ferrer-di Martino; F Gauthier; T Moreau
Journal:  Biochem J       Date:  1998-04-01       Impact factor: 3.857

2.  Identification of a new class of nonpeptidic inhibitors of cruzain.

Authors:  Katrien Brak; Patricia S Doyle; James H McKerrow; Jonathan A Ellman
Journal:  J Am Chem Soc       Date:  2008-04-25       Impact factor: 15.419

3.  Cathepsin V is involved in the degradation of invariant chain in human thymus and is overexpressed in myasthenia gravis.

Authors:  Eva Tolosa; Weijie Li; Yoshiyuki Yasuda; Wolfgang Wienhold; Lisa K Denzin; Alfred Lautwein; Christoph Driessen; Petra Schnorrer; Ekkehard Weber; Stefan Stevanovic; Raffael Kurek; Arthur Melms; Dieter Bromme
Journal:  J Clin Invest       Date:  2003-08       Impact factor: 14.808

4.  Role for cathepsin F in invariant chain processing and major histocompatibility complex class II peptide loading by macrophages.

Authors:  G P Shi; R A Bryant; R Riese; S Verhelst; C Driessen; Z Li; D Bromme; H L Ploegh; H A Chapman
Journal:  J Exp Med       Date:  2000-04-03       Impact factor: 14.307

5.  Degradation of mouse invariant chain: roles of cathepsins S and D and the influence of major histocompatibility complex polymorphism.

Authors:  J A Villadangos; R J Riese; C Peters; H A Chapman; H L Ploegh
Journal:  J Exp Med       Date:  1997-08-18       Impact factor: 14.307

Review 6.  Proteolysis and antigen presentation by MHC class II molecules.

Authors:  Paula Wolf Bryant; Ana-Maria Lennon-Duménil; Edda Fiebiger; Cécile Lagaudrière-Gesbert; Hidde L Ploegh
Journal:  Adv Immunol       Date:  2002       Impact factor: 3.543
  6 in total

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