| Literature DB >> 8844616 |
E Hulier1, P Pétour, M Marussig, M P Nivez, D Mazier, L Rénia.
Abstract
In vitro amplification of DNA by PCR is a powerful tool to detect small amounts of DNA. It is now widely used for detection of pathogenic agents from extracellular fluids and organs. The use of anion exchange HPLC to quantify the PCR product resulting from the specific amplification of the DNA from the replicative-defective viral DNA responsible for MAIDS is described. This technique allows precise quantification of MAIDS virus DNA in different organs and circumvents the use of radioactivity and gel electrophoresis.Entities:
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Year: 1996 PMID： 8844616 DOI： 10.1016/0166-0934(96)02017-4
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014