Literature DB >> 8844312

Twelve-well culture plate for the efficient collection and culture of human oocytes and embryos.

K C Drury1, J R Konicek, S Kipersztok, R S Williams.   

Abstract

OBJECTIVE: Our goal was to assess a 12-well oocyte collection and embryo culture plate for use in the IVF laboratory.
DESIGN: This was a prospective nonrandomized study.
SETTING: The setting was a university in vitro fertilization program. PATIENTS: Eighty-four consecutive infertility couples presenting for IVF were studied. MAIN OUTCOME MEASURES: The main outcome measure was pregnancy (delivered).
RESULTS: A 34% delivered pregnancy rate per retrieval was attained using the 12-well collection and culture plate without the use of expensive culture media and special serum supplementation.
CONCLUSIONS: The use of a 12-well plate for oocyte collection and embryo culture provides a simple, economical, efficient, and effective means of producing human embryos during in vitro fertilization. This system is capable of supporting high rates of ongoing and delivered pregnancies.

Entities:  

Mesh:

Year:  1996        PMID: 8844312     DOI: 10.1007/bf02066608

Source DB:  PubMed          Journal:  J Assist Reprod Genet        ISSN: 1058-0468            Impact factor:   3.412


  7 in total

1.  Use of coculture with cumulus cells in insemination medium in human in vitro fertilization (IVF).

Authors:  P Quinn
Journal:  J Assist Reprod Genet       Date:  1994-05       Impact factor: 3.412

2.  In vitro deleterious effect of hypoxanthine in Ham's Nutrient Mixture F-10 culture medium on human oocyte fertilization and early embryonic development.

Authors:  M C Bastias; S T McGee-Belser; S H Bryan; J M Vasquez
Journal:  Fertil Steril       Date:  1993-11       Impact factor: 7.329

3.  Coculture of human zygotes on fetal bovine uterine fibroblasts: embryonic morphology and implantation.

Authors:  K E Wiemer; J Cohen; S R Wiker; H E Malter; G Wright; R A Godke
Journal:  Fertil Steril       Date:  1989-09       Impact factor: 7.329

Review 4.  Radical solutions and cultural problems: could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro?

Authors:  M H Johnson; M H Nasr-Esfahani
Journal:  Bioessays       Date:  1994-01       Impact factor: 4.345

5.  High continuing pregnancy rates after in vitro fertilization-embryo transfer using medium supplemented with a plasma protein fraction containing alpha- and beta-globulins.

Authors:  T B Pool; J E Martin
Journal:  Fertil Steril       Date:  1994-04       Impact factor: 7.329

6.  The developmental potential of mouse embryos conceived in Ham's F-10 medium containing ethylenediaminetetraacetic acid.

Authors:  T S Mehta; A A Kiessling
Journal:  Fertil Steril       Date:  1993-12       Impact factor: 7.329

7.  An improved medium for long-term culture of human embryos overcomes the in vitro developmental block and increases blastocyst formation.

Authors:  L FitzGerald; M DiMattina
Journal:  Fertil Steril       Date:  1992-03       Impact factor: 7.329

  7 in total
  1 in total

1.  Normal twins born following preimplantation genetic diagnosis in a couple affected by X-linked hydrocephaly.

Authors:  K C Drury; T K Schalue; G F Pitcher; S Kipersztok; R S Williams; J R Konicek
Journal:  J Assist Reprod Genet       Date:  1996-11       Impact factor: 3.412

  1 in total

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