W Hooe1, B Steinberg. 1. Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, USA.
Abstract
OBJECTIVES: This study tested various protocols for bone decontamination after bacterial contact to determine if these treatments altered bone structure. STUDY DESIGN: Femurs from five Sprague-Dawley rats were sectioned and separated into eight groups. These were contaminated in a broth containing Pseudomonas aeroginosa and Staphylococcus aureus. Subsequently, the groups were treated with eight different decontamination regimens. A Scheffe's Grouping test was used to statistically compare the bacterial counts after each treatment protocol. RESULTS: Treatment with 4% chlorhexidine gluconate and 4% alcohol, Neosporin, cefazolin, and saline solution had little effect on bacterial growth. However, povidone-iodine, autoclaving, and ethyl alcohol with ethanol did significantly decrease the bacterial colony counts from the bone specimens. The autoclave and ethyl alcohol/ethanol induced changes in bony histologic examination. CONCLUSIONS: Results suggested that povidone-iodine decontaminates bone specimens without altering histologic conditions. Determination of successful grafting of bone treated with this protocol is required before its recommendation for clinical use.
OBJECTIVES: This study tested various protocols for bone decontamination after bacterial contact to determine if these treatments altered bone structure. STUDY DESIGN: Femurs from five Sprague-Dawley rats were sectioned and separated into eight groups. These were contaminated in a broth containing Pseudomonas aeroginosa and Staphylococcus aureus. Subsequently, the groups were treated with eight different decontamination regimens. A Scheffe's Grouping test was used to statistically compare the bacterial counts after each treatment protocol. RESULTS: Treatment with 4% chlorhexidine gluconate and 4% alcohol, Neosporin, cefazolin, and saline solution had little effect on bacterial growth. However, povidone-iodine, autoclaving, and ethyl alcohol with ethanol did significantly decrease the bacterial colony counts from the bone specimens. The autoclave and ethyl alcohol/ethanol induced changes in bony histologic examination. CONCLUSIONS: Results suggested that povidone-iodine decontaminates bone specimens without altering histologic conditions. Determination of successful grafting of bone treated with this protocol is required before its recommendation for clinical use.
Authors: M F Mat-Salleh; A N Sadagatullah; M Y Ibrahim; I Abdul-Aziz; W A Wan-Abdullah; N Maning; M N Md-Hassan; M R Ab-Rashid Journal: Malays Orthop J Date: 2021-07