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Literature DB >> 8842244

Scanning tunneling microscopy of mercapto-hexyl-oligonucleotides attached to gold.

D Rekesh¹, Y Lyubchenko, L S Shlyakhtenko, S M Lindsay.

Abstract

6-mercapto hexyl-oligonucleotides bind to a gold surface strongly enough to permit imaging by a scanning tunneling microscope (STM). STM images showed worm-like chains that were approximately 12-(A-wide for single-stranded DNA and 20-(A-wide for double-stranded DNA. The chain lengths corresponded to 3.4 +/- 0.4 A per basepair for double-stranded DNA and 2.2 +/- 0.4 A per base for single-stranded DNA. This unexpectedly short length for single-stranded DNA was confirmed using oligomers with both single- and double-stranded regions. When the attachment of the samples was weakened (by imaging in water or scraping with the STM tip) the images changed to pairs of "blobs," apparently reflecting the attachment points of the molecules to the gold surface. Given this interpretation, images of DNA containing a five-base bulge imply that the bulge bends the oligomer by 90 degrees +/- 20 degrees.

Entities: Chemical

Mesh：

Substances：

Year: 1996 PMID： 8842244 PMCID： PMC1233562 DOI： 10.1016/S0006-3495(96)79308-6

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

21 in total

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Authors: Y L Lyubchenko; A A Gall; L S Shlyakhtenko; R E Harrington; B L Jacobs; P I Oden; S M Lindsay
Journal: J Biomol Struct Dyn Date: 1992-12

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Journal: Ultramicroscopy Date: 1990-08 Impact factor: 2.689

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Journal: Phys Rev Lett Date: 1991-03-04 Impact factor: 9.161

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Authors: Y L Lyubchenko; B L Jacobs; S M Lindsay; A Stasiak
Journal: Scanning Microsc Date: 1995-09

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Authors: C H Hsieh; J D Griffith
Journal: Proc Natl Acad Sci U S A Date: 1989-07 Impact factor: 11.205

6. Molecular and thermodynamic properties of d(A(+)-G)10, a single-stranded nucleic acid helix without paired or stacked bases.

Authors: N G Dolinnaya; E H Braswell; J A Fossella; H Klump; J R Fresco
Journal: Biochemistry Date: 1993-09-28 Impact factor: 3.162

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Authors: D D Dunlap; R García; E Schabtach; C Bustamante
Journal: Proc Natl Acad Sci U S A Date: 1993-08-15 Impact factor: 11.205

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Authors: T Thundat; D P Allison; R J Warmack
Journal: Nucleic Acids Res Date: 1994-10-11 Impact factor: 16.971

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Authors: M Hegner; P Wagner; G Semenza
Journal: FEBS Lett Date: 1993-12-28 Impact factor: 4.124

10. The synthesis of protected 5'-mercapto-2',5'-dideoxyribonucleoside-3'-O-phosphoramidites; uses of 5'-mercapto-oligodeoxyribonucleotides.

Authors: B S Sproat; B Beijer; P Rider; P Neuner
Journal: Nucleic Acids Res Date: 1987-06-25 Impact factor: 16.971

6 in total

6. Immobilization, hybridization, and oxidation of synthetic DNA on gold surface: electron transfer investigated by electrochemistry and scanning tunneling microscopy.

Authors: Gerald D McEwen; Fan Chen; Anhong Zhou
Journal: Anal Chim Acta Date: 2009-04-08 Impact factor: 6.558