Transcription of mutS and mutL-homologous genes in Saccharomyces cerevisiae during the cell cycle.

Transcription of the Saccharomyces cerevisiae DNA mismatch repair genes PMS1, MSH2, and MSH6, a recently discovered homolog of the Escherichia coli mutS gene, was shown to be cell cycle regulated. In contrast, transcription of the MSH1, MSH3 and MLH1 genes was not regulated during the cell cycle. The MSH1 gene, which is thought to be involved in DNA mismatch repair in mitochondria, was also not induced under aerobic growth conditions. Regulation of the PMS1 gene was dependent on intact MluI cell cycle boxes, as demonstrated by analysis of a promoter mutant. Both reduced and increased expression of PMS1 resulted in a mitotic mutator phenotype. Analysis of mRNA levels was performed with a newly developed reverse transcription-PCR (polymerase chain reaction) approach using fluorescently labeled primers and an automated DNA sequencer for detection of PCR products.