BACKGROUND: Major cat allergen Fel d I is produced consistently by skin and by sebaceous glands before being spread on the fur. OBJECTIVE: Since cats have tubular anal glands secreting sebum, proteins and lipids, we looked at the possible presence of Fel d I in these secretions and compared the levels found to those already reported in other cat tissues or secretions. METHODS: Thirty-seven cats were studied. Fel d I dosage in the anal sacs' secretions was performed using an enzyme linked immunosorbent assay (ELISA) method and total protein evaluation by the Bradford's method. RESULTS: The geometric mean Fel d I concentration was 41 U/g secretion which represents 3.4% of the total protein levels. This amount is the highest ever reported in cat tissues or secretions. CONCLUSION: The close association of Fel d I protein with skin sebaceous glands and anal sacs both with holocrine function and lipids' secretions in one hand, and the homology of chain I of Fel d I with some steroid-binding proteins in other hand, suggest a possible physiological role for Fel d I in the regulation of lipids on skin and cat fur.
BACKGROUND: Major cat allergen Fel d I is produced consistently by skin and by sebaceous glands before being spread on the fur. OBJECTIVE: Since cats have tubular anal glands secreting sebum, proteins and lipids, we looked at the possible presence of Fel d I in these secretions and compared the levels found to those already reported in other cat tissues or secretions. METHODS: Thirty-seven cats were studied. Fel d I dosage in the anal sacs' secretions was performed using an enzyme linked immunosorbent assay (ELISA) method and total protein evaluation by the Bradford's method. RESULTS: The geometric mean Fel d I concentration was 41 U/g secretion which represents 3.4% of the total protein levels. This amount is the highest ever reported in cat tissues or secretions. CONCLUSION: The close association of Fel d I protein with skin sebaceous glands and anal sacs both with holocrine function and lipids' secretions in one hand, and the homology of chain I of Fel d I with some steroid-binding proteins in other hand, suggest a possible physiological role for Fel d I in the regulation of lipids on skin and cat fur.
Authors: Erich Knop; Nadja Knop; Thomas Millar; Hiroto Obata; David A Sullivan Journal: Invest Ophthalmol Vis Sci Date: 2011-03-30 Impact factor: 4.799
Authors: P S Tsai; J E Evans; K M Green; R M Sullivan; D A Schaumberg; S M Richards; M R Dana; D A Sullivan Journal: Br J Ophthalmol Date: 2006-03 Impact factor: 4.638
Authors: Franziska Thoms; Stefanie Haas; Aline Erhart; Claudia S Nett; Silvia Rüfenacht; Nicole Graf; Arnis Strods; Gauravraj Patil; Thonur Leenadevi; Michael C Fontaine; Lindsey A Toon; Gary T Jennings; Gabriela Senti; Thomas M Kündig; Martin F Bachmann Journal: Viruses Date: 2020-03-06 Impact factor: 5.048