BACKGROUND: This study evaluated the specific subset composition of lymphocytes present in Helicobacter pylori-associated gastritis (HAG), since reports so far have led to inconclusive and conflicting results. METHODS: Endoscopic biopsy specimens from 56 patients were studied by quantitative immunohistochemistry using monoclonal antibodies against the lymphocyte markers CD3, CD22, CD4, CD8, CD45RO, CD1O3, TCR alpha/beta, and TCR gamma/delta. Lamina propria and intraepithelial compartments were evaluated separately. Grade, activity, and density of bacterial colonisation were assessed histologically and with Warthin-Starry stain. RESULTS: Evaluation showed a significant increase in CD4+, CD45RO+, TCR alpha/beta+ activated lamina propria lymphocytes in HAG which correlated with grade and activity of gastritis and degree of bacterial colonisation, whereas subsets of intraepithelial lymphocytes did not change significantly. TCR gamma/delta+ T cells were not found to be increased in HAG. CONCLUSIONS: Selective expansion of CD4+, CD45RO+ memory-type T cells could reflect an antigen-specific and/or chemokine-mediated effect in HAG. Locally produced CC chemokines such as RANTES have been detected in HAG.
BACKGROUND: This study evaluated the specific subset composition of lymphocytes present in Helicobacter pylori-associated gastritis (HAG), since reports so far have led to inconclusive and conflicting results. METHODS: Endoscopic biopsy specimens from 56 patients were studied by quantitative immunohistochemistry using monoclonal antibodies against the lymphocyte markers CD3, CD22, CD4, CD8, CD45RO, CD1O3, TCR alpha/beta, and TCR gamma/delta. Lamina propria and intraepithelial compartments were evaluated separately. Grade, activity, and density of bacterial colonisation were assessed histologically and with Warthin-Starry stain. RESULTS: Evaluation showed a significant increase in CD4+, CD45RO+, TCR alpha/beta+ activated lamina propria lymphocytes in HAG which correlated with grade and activity of gastritis and degree of bacterial colonisation, whereas subsets of intraepithelial lymphocytes did not change significantly. TCR gamma/delta+ T cells were not found to be increased in HAG. CONCLUSIONS: Selective expansion of CD4+, CD45RO+ memory-type T cells could reflect an antigen-specific and/or chemokine-mediated effect in HAG. Locally produced CC chemokines such as RANTES have been detected in HAG.
Authors: Anna Lundgren; Christina Trollmo; Anders Edebo; Ann-Mari Svennerholm; B Samuel Lundin Journal: Infect Immun Date: 2005-09 Impact factor: 3.441
Authors: K Uchida; K Okazaki; A Debrecceni; T Nishi; H Iwano; M Inai; S Uose; H Nakase; M Ohana; C Oshima; Y Matsushima; C Kawanami; H Hiai; T Masuda; T Chiba Journal: Infect Immun Date: 2001-11 Impact factor: 3.441
Authors: Elzbieta Maciorkowska; Maciej Kaczmarski; Anatol Panasiuk; Katarzyna Kondej-Muszynska; Andrzej Kemonai Journal: World J Gastroenterol Date: 2005-11-21 Impact factor: 5.742
Authors: Mai Ping Tan; John Pedersen; Yifan Zhan; Andrew M Lew; Martin J Pearse; Odilia L C Wijburg; Richard A Strugnell Journal: Infect Immun Date: 2007-11-19 Impact factor: 3.441