Literature DB >> 8830500

Identification and characterization of the porcine reproductive and respiratory virus ORFs 7, 5 and 4 products.

S Mounir1, H Mardassi, S Dea.   

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes abortions and respiratory diseases in pigs. The PRRSV genome is a positive-sense polyadenylated RNA molecule of about 15 kb. Along with the genes for structural proteins (envelope, matrix and nucleocapsid proteins) PRRSV genome contains a number of ORFs potentially encoding nonstructural proteins (ns). To investigate the nature of the PRRSV ORFs 7, 5 and 4 products, we have cloned the envelope (ORF5) nucleocapsid (ORF7) and ns4 (ORF4) protein genes in the bacterial expression vector pMAL(TM)-c2 under control of the "tac" promoter and expressed the proteins in E. coli. The recombinant proteins were recognized by porcine and rabbit hyperimmune serums to PRRSV, suggesting their structural nature.

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Year:  1995        PMID： 8830500     DOI： 10.1007/978-1-4615-1899-0_51

Source DB:  PubMed          Journal:  Adv Exp Med Biol        ISSN： 0065-2598            Impact factor:   2.622


  10 in total

1.  A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: expression of green fluorescent protein in the Nsp2 region.

Authors:  Ying Fang; Raymond R R Rowland; Michael Roof; Joan K Lunney; Jane Christopher-Hennings; Eric A Nelson
Journal:  J Virol       Date:  2006-09-13       Impact factor: 5.103

2.  Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein.

Authors:  Mingeun Sagong; Choi Kyu Park; Seong Hee Kim; Sung Up Moon; Seong Cheol Cho; Changhee Lee
Journal:  J Vet Sci       Date:  2010-06       Impact factor: 1.672

3.  Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents.

Authors:  C J Nelsen; M P Murtaugh; K S Faaberg
Journal:  J Virol       Date:  1999-01       Impact factor: 5.103

4.  Antibody response to porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural proteins and implications for diagnostic detection and differentiation of PRRSV types I and II.

Authors:  Elizabeth Brown; Steven Lawson; Craig Welbon; Josephine Gnanandarajah; Juan Li; Michael P Murtaugh; Eric A Nelson; Ramon M Molina; Jeffery J Zimmerman; Raymond R R Rowland; Ying Fang
Journal:  Clin Vaccine Immunol       Date:  2009-03-04

5.  Mutations in a Highly Conserved Motif of nsp1β Protein Attenuate the Innate Immune Suppression Function of Porcine Reproductive and Respiratory Syndrome Virus.

Authors:  Yanhua Li; Duan-Liang Shyu; Pengcheng Shang; Jianfa Bai; Kang Ouyang; Santosh Dhakal; Jagadish Hiremath; Basavaraj Binjawadagi; Gourapura J Renukaradhya; Ying Fang
Journal:  J Virol       Date:  2016-01-20       Impact factor: 5.103

6.  Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States.

Authors:  Susan L Ropp; Carrie E Mahlum Wees; Ying Fang; Eric A Nelson; Kurt D Rossow; Melissa Bien; Bill Arndt; Sarah Preszler; Pamela Steen; Jane Christopher-Hennings; James E Collins; David A Benfield; Kay S Faaberg
Journal:  J Virol       Date:  2004-04       Impact factor: 5.103

7.  Antigenic variability among North American and European strains of porcine reproductive and respiratory syndrome virus as defined by monoclonal antibodies to the matrix protein.

Authors:  S Dea; C A Gagnon; H Mardassi; G Milane
Journal:  J Clin Microbiol       Date:  1996-06       Impact factor: 5.948

8.  Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors.

Authors:  Byoung-Joo Seo; Hyunil Kim; Ho-Seong Cho; Byoung-Yong Park; Won-Il Kim
Journal:  J Vet Med Sci       Date:  2015-08-20       Impact factor: 1.267

9.  Preparation of North American type II PRRSV infectious clone expressing green fluorescent protein.

Authors:  Liyue Wang; Kao Zhang; Hongyu Lin; Wenyan Li; Jiexia Wen; Jianlou Zhang; Yonghong Zhang; Xiujin Li; Fei Zhong
Journal:  Biomed Res Int       Date:  2014-05-08       Impact factor: 3.411

10.  Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus.

Authors:  Dandan Yu; Jian Lv; Zhi Sun; Haihong Zheng; Jiaqi Lu; Shishan Yuan
Journal:  Virology       Date:  2008-11-05       Impact factor: 3.616
  10 in total

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