Literature DB >> 8828134

Improved method for preparation of samples for the polymerase chain reaction for detection of Coxiella burnetii in milk using immunomagnetic separation.

Y Muramatsu1, M Maruyama, T Yanase, H Ueno, C Morita.   

Abstract

Immunomagnetic separation (IMS) improved the detection of Coxiella burnetii in cow's milk by the polymerase chain reaction (PCR). IMS represents a novel approach to the isolation of microorganism from milk. The combination of IMS and PCR analysis has an adequate sensitivity for detection of C. burnetii.

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Year:  1996        PMID： 8828134     DOI： 10.1016/0378-1135(95)00203-0

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  3 in total

1.  Detection of Coxiella burnetii in cow's milk by PCR-enzyme-linked immunosorbent assay combined with a novel sample preparation method.

Authors:  Y Muramatsu; T Yanase; T Okabayashi; H Ueno; C Morita
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

2.  Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation.

Authors:  I R Grant; H J Ball; M T Rowe
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

3.  PCR detection of Coxiella burnetii from different clinical specimens, especially bovine milk, on the basis of DNA preparation with a silica matrix.

Authors:  H Lorenz; C Jäger; H Willems; G Baljer
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792
  3 in total

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