Application of a modified disc diffusion technique to antimicrobial susceptibility testing of Vibrio anguillarum and Aeromonas salmonicida clinical isolates.

Two techniques for antimicrobial susceptibility testing of Vibrio anguillarum and Aeromonas salmonicida strains were compared. The first method was the reference test that determines Minimal Inhibitory Concentrations (MIC); the second was a modified diffusion test that measures the Inhibitory Concentrations in Diffusion (ICD) by carrying out the diffusion test with five discs of differing contents. ICD measurement was not applicable for the susceptibility testing of oxytetracycline and sulfadimethoxine. On the other hand, a good correlation between the MICs and the ICDs was observed for oxolinic acid, sarafloxacin, chloramphenicol and trimethoprim. Moreover, the ICD values were close to those obtained for the MIC values. A. salmonicida resistant strains were detected by ICD determination. Thus, ICD could be used instead of MIC for oxolinic acid, sarafloxacin, trimethoprim and chloramphenicol susceptibility testings. The ICD technique is easy to carry out and is not dependent on the growth characteristics of bacteria.