T cell mediation of abnormally low production of ovalbumin-specific immunoglobulin A in patients allergic to eggs.

Cells producing IgA specific to ovalbumin (OVA) were detected with an assay of plaque-forming cells (PFC). Non-T cells were separated on a polystyrene resin column and were further depleted of B cells that bound sheep erythrocytes (SRBC) by SRBC-rosette sedimentation. The cells were recombined with T cells separated on a polystyrene resin column, stimulated with OVA antigen, and then cultured for 5 d. The number of OVA-specific IgA-PFC from the lymphocytes of infants allergic to hen's eggs (7 +/- 5 per 7 x 10(4) non-T cells, n = 9) was significantly less than that of PFC from lymphocytes of age-matched controls (110 +/- 18 per 7 x 10(4) non-T cells, n = 7) and from those of children with atopic dermatitis who were not allergic to hen's eggs (90 +/- 30 per 7 x 10(4) non-T cells, n = 4). Patients' B cells added to the culture supernatant from OVA-stimulated normal T cells (82 +/- 18 per 7 x 10(4) non-T cells, n = 4) were able to produce the specific IgA to levels comparable to those of normal B cells (92 +/- 9 per 7 x 10(4) non-T cells, n = 6), but the patients' T cells did not cause normal B cells to produce the antibody (8 +/- 2 per 7 x 10(4) non-T cells, n = 4). This indicates that the patients' T cells were less able than were normal T cells to promote the production of OVA-specific IgA-PFC. Until the age of 6 y, the ability of the patients' lymphocytes to produce specific IgA was abnormally low; from that age on, it was normal. At the stage of allergen entry, this transiently low production of OVA-specific IgA may contribute to the onset of allergy to hen's eggs.