| Literature DB >> 8825762 |
A Rayms-Keller1, A M Powers, S Higgs, K E Olson, K I Kamrud, J O Carlson, B J Beaty.
Abstract
A recombinant Sindbis virus, TE/3'2J/ANTI-S, containing LaCrosse virus small segment cDNA in antisense orientation, was inoculated into Aedes triseriatus mosquitoes. Virus replication and LAC-ANTI-S RNA expression were analysed temporally and spatially. TE/3'2J/ANTI-S virus titre peaked at 5.0 log10 TCID50 in heads 6-9 days post infection (p.i.) and decreased to 3.4 log10 TCID50 by 37 days p.i. Salivary glands contained 4.4 log10 TCID50 of virus 6 days p.i.; titres were lower in other organs. LAC-ANTI-S RNA levels paralleled virus titre. SIN E1 antigen was detected in many mosquito organ systems, but in specific cells and tissues of some organs. TE/3'2J/ANTI-S virus exhibited different cellular tropisms in salivary glands of Aedes and Culex mosquitoes.Entities:
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Year: 1995 PMID： 8825762 DOI： 10.1111/j.1365-2583.1995.tb00030.x
Source DB: PubMed Journal: Insect Mol Biol ISSN： 0962-1075 Impact factor: 3.585