INTRODUCTION: Acetylcholine (ACh) is a well known neurotransmitter in the central nervous system, but determining its level in cerebrospinal fluid (CSF) is very difficult and the origin of CSF ACh is still unknown. In this study, we attempted to measure CSF ACh by a specific and sensitive radioimmunoassay (RIA) from patients with neurologic diseases. MATERIAL AND METHODS: Patients with cerebral infarction (n = 7), Parkinson's disease (n = 6), spinocerebellar degeneration (n = 6), Alzheimer's disease (n = 3), amyotrophic lateral sclerosis (n = 3) and disc herniation with no central nervous involvement (n = 8) participated to determine the CSF ACh levels. RESULTS: Of these 33 patients, the mean ACh level in CSF was 282.2 +/- 61.7 fmol/ml (mean +/- SE, range 20-1505.8 fmol/ml). The mean ACh level of spinocerebellar degeneration group was lower than others, but not statistically significant. CONCLUSION: We conclude that an amount of ACh detectable by RIA is certainly present in CSF.
INTRODUCTION:Acetylcholine (ACh) is a well known neurotransmitter in the central nervous system, but determining its level in cerebrospinal fluid (CSF) is very difficult and the origin of CSF ACh is still unknown. In this study, we attempted to measure CSF ACh by a specific and sensitive radioimmunoassay (RIA) from patients with neurologic diseases. MATERIAL AND METHODS:Patients with cerebral infarction (n = 7), Parkinson's disease (n = 6), spinocerebellar degeneration (n = 6), Alzheimer's disease (n = 3), amyotrophic lateral sclerosis (n = 3) and disc herniation with no central nervous involvement (n = 8) participated to determine the CSF ACh levels. RESULTS: Of these 33 patients, the mean ACh level in CSF was 282.2 +/- 61.7 fmol/ml (mean +/- SE, range 20-1505.8 fmol/ml). The mean ACh level of spinocerebellar degeneration group was lower than others, but not statistically significant. CONCLUSION: We conclude that an amount of ACh detectable by RIA is certainly present in CSF.
Authors: David R Linden; Michael D Levitt; Gianrico Farrugia; Joseph H Szurszewski Journal: Antioxid Redox Signal Date: 2010-05-01 Impact factor: 8.401
Authors: Félix J Jiménez-Jiménez; Hortensia Alonso-Navarro; Elena García-Martín; José A G Agúndez Journal: Front Cell Neurosci Date: 2014-11-11 Impact factor: 5.505
Authors: Marcella Reale; Federica de Angelis; Marta di Nicola; Elisabetta Capello; Maria di Ioia; Giovanna de Luca; Alessandra Lugaresi; Ada Maria Tata Journal: Int J Mol Sci Date: 2012-10-03 Impact factor: 5.923