Literature DB >> 8824627

Effect of traY amber mutations on F-plasmid traY promoter activity in vivo.

P M Silverman1, A Sholl.   

Abstract

We have examined the effect of the F plasmid TraY protein on tra gene expression in vivo. Expression was assayed as alkaline phosphatase activity in cells containing a traY phi(traA'-'phoA)hyb operon under traY promoter control. Amber mutations in traY significantly reduced alkaline phosphatase activity. Since nonsense polarity effects were minimal, if they occurred at all, these data provide the first direct evidence that TraY regulates tra gene expression.

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Year:  1996        PMID： 8824627      PMCID： PMC178421          DOI： 10.1128/jb.178.19.5787-5789.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  26 in total

1.  Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.

Authors:  E Brickman; J Beckwith
Journal:  J Mol Biol       Date:  1975-08-05       Impact factor: 5.469

2.  TraY proteins of F and related episomes are members of the Arc and Mnt repressor family.

Authors:  J U Bowie; R T Sauer
Journal:  J Mol Biol       Date:  1990-01-05       Impact factor: 5.469

3.  Specific binding of the TraY protein to oriT and the promoter region for the traY gene of plasmid R100.

Authors:  S Inamoto; E Ohtsubo
Journal:  J Biol Chem       Date:  1990-04-15       Impact factor: 5.157

Review 4.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

5.  Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.

Authors:  M Achtman; N Willetts; A J Clark
Journal:  J Bacteriol       Date:  1971-05       Impact factor: 3.490

6.  Characterisation of an in vivo system for nicking at the origin of conjugal DNA transfer of the sex factor F.

Authors:  R Everett; N Willetts
Journal:  J Mol Biol       Date:  1980-01-15       Impact factor: 5.469

7.  Regulation of the F conjugation genes studied by hybridization and tra-lacZ fusion.

Authors:  D Gaffney; R Skurray; N Willetts
Journal:  J Mol Biol       Date:  1983-07-25       Impact factor: 5.469

8.  Structure and function of conjugative pili: inducible synthesis of functional F pili by Escherichia coli K-12 containing a lac-tra operon fusion.

Authors:  T H Grossman; P M Silverman
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490

9.  Structure of Arc repressor in solution: evidence for a family of beta-sheet DNA-binding proteins.

Authors:  J N Breg; J H van Opheusden; M J Burgering; R Boelens; R Kaptein
Journal:  Nature       Date:  1990-08-09       Impact factor: 49.962

10.  Stepwise assembly of a relaxosome at the F plasmid origin of transfer.

Authors:  M T Howard; W C Nelson; S W Matson
Journal:  J Biol Chem       Date:  1995-11-24       Impact factor: 5.157
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  7 in total

1.  Characterization of the opposing roles of H-NS and TraJ in transcriptional regulation of the F-plasmid tra operon.

Authors:  William R Will; Laura S Frost
Journal:  J Bacteriol       Date:  2006-01       Impact factor: 3.490

2.  TraY and integration host factor oriT binding sites and F conjugal transfer: sequence variations, but not altered spacing, are tolerated.

Authors:  Sarah L Williams; Joel F Schildbach
Journal:  J Bacteriol       Date:  2007-03-09       Impact factor: 3.490

3.  Transfer protein TraY of plasmid R1 stimulates TraI-catalyzed oriT cleavage in vivo.

Authors:  W Karl; M Bamberger; E L Zechner
Journal:  J Bacteriol       Date:  2001-02       Impact factor: 3.490

4.  In vitro analysis of protein-operator interactions of the NikR and fur metal-responsive regulators of coregulated genes in Helicobacter pylori.

Authors:  Isabel Delany; Raffaele Ieva; Alice Soragni; Markus Hilleringmann; Rino Rappuoli; Vincenzo Scarlato
Journal:  J Bacteriol       Date:  2005-11       Impact factor: 3.490

5.  Regulation of bacterial conjugation in microaerobiosis by host-encoded functions ArcAB and sdhABCD.

Authors:  Ana Serna; Elena Espinosa; Eva M Camacho; Josep Casadesús
Journal:  Genetics       Date:  2010-01-18       Impact factor: 4.562

6.  Investigating the basis of substrate recognition in the pC221 relaxosome.

Authors:  Jamie A Caryl; Christopher D Thomas
Journal:  Mol Microbiol       Date:  2006-06       Impact factor: 3.501

7.  MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer.

Authors:  Jolanta Godziszewska; Anna Kulińska; Grażyna Jagura-Burdzy
Journal:  BMC Microbiol       Date:  2014-09-04       Impact factor: 3.605
  7 in total

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