| Literature DB >> 8824229 |
Abstract
The large N-linked oligosaccharides released by endo-beta-N-acetylglucosaminidase H from Schizosaccharomyces pombe glycoproteins were analyzed for the presence of noncarbohydrate functional groups. No phosphate, sulfate, or acetate could be detected; however, approximately six molecules of pyruvic acid/molecule were found on 98% of the oligosaccharides. Pyruvate moieties were acetal (ketal)-linked to galactose residues in the R configuration to carbons 4 and 6. This is the first report of pyruvate functional groups being attached to N-linked oligosaccharides in yeast and appears only to be the second documentation of this sugar modification in eukaryotes.Entities:
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Year: 1996 PMID: 8824229 DOI: 10.1074/jbc.271.42.25945
Source DB: PubMed Journal: J Biol Chem ISSN: 0021-9258 Impact factor: 5.157