PURPOSE: To characterize the acute and chronic behavioral, electrographic and histologic effects of sustained seizures induced by pilocarpine in mice. METHODS: After status epilepticus, the surviving animals were continuously monitored for 24 h/day for < or = 120 days. The brains were processed by using neo-Timm and Nissl stains. RESULTS: The first spontaneous seizures occurred between 4 and 42 days after status epilepticus. The mean "seizure-silent period" lasted for 14.4 +/- 11.9 days. During the chronic phase, recurrent spontaneous seizures were observed 1-5 times per animal per week and were associated with sprouting in the supragranular layer of the dentate gyrus. CONCLUSIONS: Structural brain damage promoted by pilocarpine-induced status epilepticus may underlie or be associated with recurrent spontaneous seizures in mice.
PURPOSE: To characterize the acute and chronic behavioral, electrographic and histologic effects of sustained seizures induced by pilocarpine in mice. METHODS: After status epilepticus, the surviving animals were continuously monitored for 24 h/day for < or = 120 days. The brains were processed by using neo-Timm and Nissl stains. RESULTS: The first spontaneous seizures occurred between 4 and 42 days after status epilepticus. The mean "seizure-silent period" lasted for 14.4 +/- 11.9 days. During the chronic phase, recurrent spontaneous seizures were observed 1-5 times per animal per week and were associated with sprouting in the supragranular layer of the dentate gyrus. CONCLUSIONS:Structural brain damage promoted by pilocarpine-induced status epilepticus may underlie or be associated with recurrent spontaneous seizures in mice.
Authors: Kevin D Phelan; Matthew M Mock; Oliver Kretz; U Thaung Shwe; Maxim Kozhemyakin; L John Greenfield; Alexander Dietrich; Lutz Birnbaumer; Marc Freichel; Veit Flockerzi; Fang Zheng Journal: Mol Pharmacol Date: 2011-12-05 Impact factor: 4.436
Authors: Graciela Navarro Mora; Placido Bramanti; Francesco Osculati; Asmaa Chakir; Elena Nicolato; Pasquina Marzola; Andrea Sbarbati; Paolo Francesco Fabene Journal: PLoS One Date: 2009-06-02 Impact factor: 3.240