Characterization of extracellular proteases from Trametes trogii.

The peptidase activities excreted in culture broths of Trametes trogii mycelium have been identified by determining the digestion pathway of various peptides. Insulin beta-chain (30 residues), procasomorphin (10 residues) and two peptides of five residues (proctolin and thymosin alpha 1 fragment 23-27) were utilized as model substrates. Aminopeptidase, carboxypeptidase, endopeptidase and dipeptidyl aminopeptidase activities were revealed and information on their specificity was deduced. Preliminary data on the pH-dependent activity of the peptidases were also obtained by sequence analysis of the fragment mixtures produced at different pH values.