Lipofuscin accumulation in neurons with restraint stress.

Stress induced lipofuscinosis was studied in rat cerebellum. 3 month old wistar rats were subjected to restraint stress by keeping them immobile for 24, 48 and 72 hours duration. This was achieved in specially prepared cages which allowed no space for the rats to move; giving a stress to the animal. The cerebella from the stressed groups rats were removed after the experiment and were processed for fluorescent microscopical, histochemical and fluorimetric study of lipofuscin. The lipofuscin content in the Purkinje neurons was compared with that of the control rats which were of the same age, size and weight as of the experimental rats. The results showed that the lipofuscin content in the neurons of the experimental rats was more than that of the control ones. In gist, while 24 hrs. stress caused a 28.9% increase in lipofuscin content, 48 hrs. stress resulted in a 38.3% increase. This shows that restraint stress can be a good experimental model for lipofuscinogenesis and ageing studies.