Literature DB >> 8819166

Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins.

I Mingarro1, P Whitley, M A Lemmon, G von Heijne.   

Abstract

Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix-helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala-insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.

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Year:  1996        PMID： 8819166      PMCID： PMC2143459          DOI： 10.1002/pro.5560050712

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  13 in total

1.  The glycophorin A transmembrane domain dimer: sequence-specific propensity for a right-handed supercoil of helices.

Authors:  H R Treutlein; M A Lemmon; D M Engelman; A T Brünger
Journal:  Biochemistry       Date:  1992-12-29       Impact factor: 3.162

2.  Sequence specificity in the dimerization of transmembrane alpha-helices.

Authors:  M A Lemmon; J M Flanagan; H R Treutlein; J Zhang; D M Engelman
Journal:  Biochemistry       Date:  1992-12-29       Impact factor: 3.162

3.  Membrane protein folding and oligomerization: the two-stage model.

Authors:  J L Popot; D M Engelman
Journal:  Biochemistry       Date:  1990-05-01       Impact factor: 3.162

4.  Exploring the allowed sequence space of a membrane protein.

Authors:  J Wen; X Chen; J U Bowie
Journal:  Nat Struct Biol       Date:  1996-02

5.  A dimerization motif for transmembrane alpha-helices.

Authors:  M A Lemmon; H R Treutlein; P D Adams; A T Brünger; D M Engelman
Journal:  Nat Struct Biol       Date:  1994-03

6.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

7.  Glycophorin A dimerization is driven by specific interactions between transmembrane alpha-helices.

Authors:  M A Lemmon; J M Flanagan; J F Hunt; B D Adair; B J Bormann; C E Dempsey; D M Engelman
Journal:  J Biol Chem       Date:  1992-04-15       Impact factor: 5.157

8.  Consequences of amino acid insertions and/or deletions in transmembrane helix C of bacteriorhodopsin.

Authors:  T Marti; H Otto; S J Rösselet; M P Heyn; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  1992-02-15       Impact factor: 11.205

9.  Structural organization of the pentameric transmembrane alpha-helices of phospholamban, a cardiac ion channel.

Authors:  I T Arkin; P D Adams; K R MacKenzie; M A Lemmon; A T Brünger; D M Engelman
Journal:  EMBO J       Date:  1994-10-17       Impact factor: 11.598

Review 10.  The lactose permease meets Frankenstein.

Authors:  H R Kaback; S Frillingos; H Jung; K Jung; G G Privé; M L Ujwal; C Weitzman; J Wu; K Zen
Journal:  J Exp Biol       Date:  1994-11       Impact factor: 3.312
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  20 in total

1.  A new class of fusion-associated small transmembrane (FAST) proteins encoded by the non-enveloped fusogenic reoviruses.

Authors:  M Shmulevitz; R Duncan
Journal:  EMBO J       Date:  2000-03-01       Impact factor: 11.598

2.  Influence of the C-terminus of the glycophorin A transmembrane fragment on the dimerization process.

Authors:  M Orzáez; E Pérez-Payá; I Mingarro
Journal:  Protein Sci       Date:  2000-06       Impact factor: 6.725

Review 3.  How do helix-helix interactions help determine the folds of membrane proteins? Perspectives from the study of homo-oligomeric helical bundles.

Authors:  William F DeGrado; Holly Gratkowski; James D Lear
Journal:  Protein Sci       Date:  2003-04       Impact factor: 6.725

4.  Role of the coronavirus E viroporin protein transmembrane domain in virus assembly.

Authors:  Ye Ye; Brenda G Hogue
Journal:  J Virol       Date:  2007-01-17       Impact factor: 5.103

5.  Palmitoylation, membrane-proximal basic residues, and transmembrane glycine residues in the reovirus p10 protein are essential for syncytium formation.

Authors:  Maya Shmulevitz; Jayme Salsman; Roy Duncan
Journal:  J Virol       Date:  2003-09       Impact factor: 5.103

6.  Structure-based prediction of the stability of transmembrane helix-helix interactions: the sequence dependence of glycophorin A dimerization.

Authors:  K R MacKenzie; D M Engelman
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-31       Impact factor: 11.205

7.  Role of the specific amino acid sequence of the membrane-spanning domain of human immunodeficiency virus type 1 in membrane fusion.

Authors:  Kosuke Miyauchi; Jun Komano; Yoshiyuki Yokomaku; Wataru Sugiura; Naoki Yamamoto; Zene Matsuda
Journal:  J Virol       Date:  2005-04       Impact factor: 5.103

8.  Tryptophan scanning analysis of the membrane domain of CTR-copper transporters.

Authors:  Christopher J De Feo; Sara Mootien; Vinzenz M Unger
Journal:  J Membr Biol       Date:  2010-03-12       Impact factor: 1.843

9.  Characterization of the GXXXG motif in the first transmembrane segment of Japanese encephalitis virus precursor membrane (prM) protein.

Authors:  Ying-Ju Lin; Jia-Guan Peng; Suh-Chin Wu
Journal:  J Biomed Sci       Date:  2010-05-24       Impact factor: 8.410

10.  Transmembrane domains of the syndecan family of growth factor coreceptors display a hierarchy of homotypic and heterotypic interactions.

Authors:  Ian C Dews; Kevin R Mackenzie
Journal:  Proc Natl Acad Sci U S A       Date:  2007-12-19       Impact factor: 11.205
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