Literature DB >> 8818907

Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay.

M Hassan-King1, I Baldeh, R Adegbola, C Omosigho, S O Usen, A Oparaugo, B M Greenwood.   

Abstract

A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.

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Year:  1996        PMID： 8818907      PMCID： PMC229179          DOI： 10.1128/jcm.34.8.2030-2032.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  PCR for capsular typing of Haemophilus influenzae.

Authors:  T J Falla; D W Crook; L N Brophy; D Maskell; J S Kroll; E R Moxon
Journal:  J Clin Microbiol       Date:  1994-10       Impact factor: 5.948

2.  Etiology of acute lower respiratory tract infections in Gambian children: II. Acute lower respiratory tract infection in children ages one to nine years presenting at the hospital.

Authors:  I M Forgie; K P O'Neill; N Lloyd-Evans; M Leinonen; H Campbell; H C Whittle; B M Greenwood
Journal:  Pediatr Infect Dis J       Date:  1991-01       Impact factor: 2.129

3.  Etiology of acute lower respiratory tract infections in Gambian children: I. Acute lower respiratory tract infections in infants presenting at the hospital.

Authors:  I M Forgie; K P O'Neill; N Lloyd-Evans; M Leinonen; H Campbell; H C Whittle; B M Greenwood
Journal:  Pediatr Infect Dis J       Date:  1991-01       Impact factor: 2.129

4.  Importance of enteric bacteria as a cause of pneumonia, meningitis and septicemia among children in a rural community in The Gambia, West Africa.

Authors:  T J O'Dempsey; T F McArdle; N Lloyd-Evans; I Baldeh; B E Laurence; O Secka; B M Greenwood
Journal:  Pediatr Infect Dis J       Date:  1994-02       Impact factor: 2.129

5.  Diagnosis of acute bacterial pneumonia in Nigerian children. Value of needle aspiration of lung of countercurrent immunoelectrophoresis.

Authors:  M Silverman; D Stratton; A Diallo; L J Egler
Journal:  Arch Dis Child       Date:  1977-12       Impact factor: 3.791

6.  Detection of Streptococcus pneumoniae DNA in blood cultures by PCR.

Authors:  M Hassan-King; I Baldeh; O Secka; A Falade; B Greenwood
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

7.  Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy.

Authors:  P Rådström; A Bäckman; N Qian; P Kragsbjerg; C Påhlson; P Olcén
Journal:  J Clin Microbiol       Date:  1994-11       Impact factor: 5.948

8.  The etiology of lobar pneumonia in the Gambia.

Authors:  R A Wall; P T Corrah; D C Mabey; B M Greenwood
Journal:  Bull World Health Organ       Date:  1986       Impact factor: 9.408
  8 in total
  7 in total

Review 1.  The role of DNA amplification technology in the diagnosis of infectious diseases.

Authors:  M Louie; L Louie; A E Simor
Journal:  CMAJ       Date:  2000-08-08       Impact factor: 8.262

2.  PCR identification of bacteria in blood culture does not fit the daily workflow of a routine microbiology laboratory.

Authors:  Santra Karumaa; Pauliina Kärpänoja; Hannu Sarkkinen
Journal:  J Clin Microbiol       Date:  2011-12-14       Impact factor: 5.948

3.  PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis.

Authors:  Tomer Avni; Nariman Mansur; Leonard Leibovici; Mical Paul
Journal:  J Clin Microbiol       Date:  2009-12-09       Impact factor: 5.948

4.  Detection of Haemophilus influenzae type b by real-time PCR.

Authors:  Adrian Marty; Oliver Greiner; Philip J R Day; Sibylle Gunziger; Kathrin Mühlemann; David Nadal
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

5.  Rapid identification of nine microorganisms causing acute respiratory tract infections by single-tube multiplex reverse transcription-PCR: feasibility study.

Authors:  B Gröndahl; W Puppe; A Hoppe; I Kühne; J A Weigl; H J Schmitt
Journal:  J Clin Microbiol       Date:  1999-01       Impact factor: 5.948

6.  PCR-Enzyme immunoassay for detection of Streptococcus pneumoniae DNA in cerebrospinal fluid samples from patients with culture-negative meningitis.

Authors:  T Cherian; M K Lalitha; A Manoharan; K Thomas; R H Yolken; M C Steinhoff
Journal:  J Clin Microbiol       Date:  1998-12       Impact factor: 5.948

7.  Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease.

Authors:  Aquino Albino Nhantumbo; Vlademir Vicente Cantarelli; Juliana Caireão; Alcides Moniz Munguambe; Charlotte Elizabeth Comé; Gabriela do Carmo Pinto; Tomás Francisco Zimba; Inácio Mandomando; Cynthia Baltazar Semá; Cícero Dias; Milton Ozório Moraes; Eduardo Samo Gudo
Journal:  PLoS One       Date:  2015-09-22       Impact factor: 3.240
  7 in total

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