The isolation of lupine cDNA clone coding for putative cyclin protein.

The complex of p34cdc2 protein kinase and cyclin is a key regulator of eukaryotic cell division and has been mostly investigated in yeast and animals. We have isolated a cDNA clone corresponding to cyclin from yellow lupine (Lupinus luteus). The cDNA clone CycBla-ll is 1692 bp in length and encodes a protein of predicted molecular mass 48.7 kDa. The lupine cyclin-like clone contains two domains: destruction and cyclin box, responsible for the function during the cell cycle. The amino acid comparison of CycBla-ll with conserved regions of other cyclins indicates that the lupine cDNA sequence represents mitotic cyclin of type B.