BACKGROUND: Perioperative analysis and classification of vitreous cell components needs a quick method sufficient to prepare both single cells and cellular membranes. METHODS: In an attempt to clarify the usefulness of immunoimpression cytology we examined 82 eyes with proliferative vitreoretinopathy, proliferative diabetic retinopathy, perforating injuries and contusions. RESULTS: With this method it is possible to perioperatively obtain cells from surfaces of vitreous membranes as well as single cells in suspension. After staining, various cell types could be differentiated morphologically and on the basis of antibody response to cell markers. We found single cells reacting with the antibodies 27E10 (4 positive of 14 tested), vimentin (2/12), RM 3/1 (2/15), LCA (1/ 11) and, particularly, with antiproliferating cell nuclear antigen (9/18). CONCLUSION: With immunoimpression cytology it is possible to determine cell surface markers rapidly and accurately from specimens obtained at the time of vitrectomy. The clinical utility of this test will be ascertained by future studies.
BACKGROUND: Perioperative analysis and classification of vitreous cell components needs a quick method sufficient to prepare both single cells and cellular membranes. METHODS: In an attempt to clarify the usefulness of immunoimpression cytology we examined 82 eyes with proliferative vitreoretinopathy, proliferative diabetic retinopathy, perforating injuries and contusions. RESULTS: With this method it is possible to perioperatively obtain cells from surfaces of vitreous membranes as well as single cells in suspension. After staining, various cell types could be differentiated morphologically and on the basis of antibody response to cell markers. We found single cells reacting with the antibodies 27E10 (4 positive of 14 tested), vimentin (2/12), RM 3/1 (2/15), LCA (1/ 11) and, particularly, with antiproliferating cell nuclear antigen (9/18). CONCLUSION: With immunoimpression cytology it is possible to determine cell surface markers rapidly and accurately from specimens obtained at the time of vitrectomy. The clinical utility of this test will be ascertained by future studies.
Authors: R Proença; M Carvalho; D Proença; J Verissimo; I Regadas; A Travassos Journal: Graefes Arch Clin Exp Ophthalmol Date: 1994-01 Impact factor: 3.117