Literature DB >> 8817195

Comparison of an improved RAPD fingerprinting with different typing methods for discriminating clinical isolates of Staphylococcus spp.

G Damiani1, S Telecco, S Comincini, M Sironi, E Carretto, P Marone.   

Abstract

Different epidemiological markers were used to characterize 2 Staphylococcus epidermidis and 8 Staphylococcus aureus strains isolated from patients with severe infections. We compared random amplified polymorphic DNA (RAPD) fingerprints, biotypes, antibiotic assays, plasmid profiles and chromosomal DNA restriction endonuclease analysis (REA). Data analysis based on numerical taxonomy methods indicates that RAPD and REA give similar results allowing a good discrimination of the two species and of each isolate. The RAPD method is easier and faster than REA, but the reproducibility of RAPD fingerprints obtained in independent experiments can be problematic. We have found simple technical devices to improve the reproducibility of the RAPD procedure which is therefore a very useful tool in epidemiology for identification and characterization of Staphylococcus spp.

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Year:  1996        PMID： 8817195     DOI： 10.1007/bf00145502

Source DB:  PubMed          Journal:  Eur J Epidemiol        ISSN： 0393-2990            Impact factor:   8.082


  22 in total

1.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

2.  Concordant clonal delineation of methicillin-resistant Staphylococcus aureus by macrorestriction analysis and polymerase chain reaction genome fingerprinting.

Authors:  M J Struelens; R Bax; A Deplano; W G Quint; A Van Belkum
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

3.  Laboratory diagnosis of oxacillin resistance in Staphylococcus aureus by a multiplex-polymerase chain reaction assay.

Authors:  G Zambardi; M E Reverdy; S Bland; M Bes; J Freney; J Fleurette
Journal:  Diagn Microbiol Infect Dis       Date:  1994-05       Impact factor: 2.803

4.  The detection of disease clustering and a generalized regression approach.

Authors:  N Mantel
Journal:  Cancer Res       Date:  1967-02       Impact factor: 12.701

5.  Restriction endonuclease analysis of Staphylococcus epidermidis DNA may be a useful epidemiological marker.

Authors:  F Renaud; J Freney; J Etienne; M Bes; Y Brun; O Barsotti; S Andre; J Fleurette
Journal:  J Clin Microbiol       Date:  1988-09       Impact factor: 5.948

6.  Management and epidemiologic analyses of an outbreak due to methicillin-resistant Staphylococcus aureus.

Authors:  M B Goetz; M E Mulligan; R Kwok; H O'Brien; C Caballes; J P Garcia
Journal:  Am J Med       Date:  1992-06       Impact factor: 4.965

7.  Random amplified polymorphic DNA assay is less discriminant than pulsed-field gel electrophoresis for typing strains of methicillin-resistant Staphylococcus aureus.

Authors:  P Saulnier; C Bourneix; G Prévost; A Andremont
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

8.  Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains.

Authors:  A van Belkum; R Bax; P Peerbooms; W H Goessens; N van Leeuwen; W G Quint
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

9.  Outbreak of methicillin-resistant Staphylococcus aureus in a teaching hospital--epidemiological and microbiological surveillance.

Authors:  F Schumacher-Perdreau; B Jansen; H Seifert; G Peters; G Pulverer
Journal:  Zentralbl Bakteriol       Date:  1994-03

10.  Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus.

Authors:  F C Tenover; R Arbeit; G Archer; J Biddle; S Byrne; R Goering; G Hancock; G A Hébert; B Hill; R Hollis
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948
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  4 in total

1.  Small colony variant of methicillin-resistant Staphylococcus pseudintermedius ST71 presenting as a sticky phenotype.

Authors:  Vincenzo Savini; Edoardo Carretto; Ennio Polilli; Roberta Marrollo; Stella Santarone; Paolo Fazii; Domenico D'Antonio; Alexandra Rossano; Vincent Perreten
Journal:  J Clin Microbiol       Date:  2014-01-22       Impact factor: 5.948

2.  Methicillin-resistant Staphylococcus pseudintermedius infection in a bone marrow transplant recipient.

Authors:  Vincenzo Savini; Daniela Barbarini; Klaudia Polakowska; Giovanni Gherardi; Anna Białecka; Andrzej Kasprowicz; Ennio Polilli; Roberta Marrollo; Giovanni Di Bonaventura; Paolo Fazii; Domenico D'Antonio; Jacek Miedzobrodzki; Edoardo Carretto
Journal:  J Clin Microbiol       Date:  2013-03-13       Impact factor: 5.948

3.  Surgical wound infection by mannitol-nonfermenting Staphylococcus aureus after lumbar microdiscectomy.

Authors:  Vincenzo Savini; Raffaele Nigro; Roberta Marrollo; Ennio Polilli; Irma Campitelli; Roberto Buonaguidi; Paolo Fazii; Edoardo Carretto
Journal:  Int J Clin Exp Pathol       Date:  2014-04-15

4.  Multiple-locus variable number tandem repeat analysis of Staphylococcus aureus: comparison with pulsed-field gel electrophoresis and spa-typing.

Authors:  Leo M Schouls; Emile C Spalburg; Martijn van Luit; Xander W Huijsdens; Gerlinde N Pluister; Marga G van Santen-Verheuvel; Han G J van der Heide; Hajo Grundmann; Max E O C Heck; Albert J de Neeling
Journal:  PLoS One       Date:  2009-04-03       Impact factor: 3.240
  4 in total

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