Literature DB >> 8813665

The promoter for the ribosomal RNA genes of Leishmania chagasi.

L S Gay1, M E Wilson, J E Donelson.   

Abstract

A promoter for the rRNA genes of Leishmania chagasi was found to be located about 1 kb upstream of the 18S rRNA coding region and immediately downstream of 64 bp tandem repeats. Its approximate boundaries and corresponding transcription start site were determined by transient transfections and primer extension assays. This promoter for RNA polymerase I has differing activities when transfected into various Leishmania species and no activity in Trypanosoma cruzi. Its sequence has no obvious similarities with other known rRNA promoters in Trypanosomatids. Depending on the species, this promoter can be used to increase expression of a protein from a plasmid in Leishmania by as much as 45-fold over that from a plasmid lacking a promoter.

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Year:  1996        PMID： 8813665     DOI： 10.1016/0166-6851(96)02594-7

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  10 in total

1.  Diversification of function by different isoforms of conventionally shared RNA polymerase subunits.

Authors:  Sara Devaux; Steven Kelly; Laurence Lecordier; Bill Wickstead; David Perez-Morga; Etienne Pays; Luc Vanhamme; Keith Gull
Journal:  Mol Biol Cell       Date:  2007-01-31       Impact factor: 4.138

Review 2.  Ribosomal RNA gene transcription in trypanosomes.

Authors:  Roberto Hernández; Ana María Cevallos
Journal:  Parasitol Res       Date:  2014-05-15       Impact factor: 2.289

3.  DNA transformation of Leishmania infantum axenic amastigotes and their use in drug screening.

Authors:  D Sereno; G Roy; J L Lemesre; B Papadopoulou; M Ouellette
Journal:  Antimicrob Agents Chemother       Date:  2001-04       Impact factor: 5.191

4.  The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor.

Authors:  M N Schnare; J C Collings; D F Spencer; M W Gray
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

5.  The ribosomal RNA gene promoter and adjacent cis-acting DNA sequences govern plasmid DNA partitioning and stable inheritance in the parasitic protozoan Leishmania.

Authors:  Nathalie Boucher; François McNicoll; Maxime Laverdière; Annie Rochette; Marie-Noëlle Chou; Barbara Papadopoulou
Journal:  Nucleic Acids Res       Date:  2004-05-25       Impact factor: 16.971

6.  Conserved Curvature of RNA Polymerase I Core Promoter Beyond rRNA Genes: The Case of the Tritryps.

Authors:  Pablo Smircich; María Ana Duhagon; Beatriz Garat
Journal:  Genomics Proteomics Bioinformatics       Date:  2015-12-21       Impact factor: 7.691

Review 7.  Nucleolar Structure and Function in Trypanosomatid Protozoa.

Authors:  Santiago Martínez-Calvillo; Luis E Florencio-Martínez; Tomás Nepomuceno-Mejía
Journal:  Cells       Date:  2019-05-08       Impact factor: 6.600

Review 8.  Investigating immune responses to parasites using transgenesis.

Authors:  Mebrahtu G Tedla; Alison L Every; Jean-Pierre Y Scheerlinck
Journal:  Parasit Vectors       Date:  2019-06-15       Impact factor: 3.876

Review 9.  Gene expression in trypanosomatid parasites.

Authors:  Santiago Martínez-Calvillo; Juan C Vizuet-de-Rueda; Luis E Florencio-Martínez; Rebeca G Manning-Cela; Elisa E Figueroa-Angulo
Journal:  J Biomed Biotechnol       Date:  2010-02-11

Review 10.  Transgenic Leishmania and the immune response to infection.

Authors:  L Beattie; K J Evans; P M Kaye; D F Smith
Journal:  Parasite Immunol       Date:  2008-02-04       Impact factor: 2.280
  10 in total

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