Literature DB >> 8813265

Cultured skin for massive burns. A prospective, controlled trial.

A M Munster1.   

Abstract

OBJECTIVE: The author compares the outcome of therapy in patients with massive burns with or without cultured autologous epithelial autografts. SUMMARY BACKGROUND DATA: The use of cultured keratinocytes has been controversial because of poor take and fragility. There have been no prospective series comparing the use of this technology with standard burn wound coverage.
METHODS: During a 5-year period, 22 patients with an average burn size of 71.8% were treated with cultured keratinocytes and compared with a group of 42 controls with an average burn size of 61.6%.
RESULTS: There was no significant difference in age, sex distribution, or third-degree component of the burn between the two groups. There was a significantly higher incidence of smoke inhalation in the cultured epithelial autograft group. There was a reduction in mortality in the cultured epithelial autograft group compared with controls, from 48% to 14% (p < 0.007). There was no difference between the two groups in other major complications, or in readmission for breakdown.
CONCLUSIONS: Coverage of patients who sustain massive burns with cultured autologous epithelial cells is an important and significant advance in management.

Entities:  

Mesh:

Year:  1996        PMID: 8813265      PMCID: PMC1235384          DOI: 10.1097/00000658-199609000-00013

Source DB:  PubMed          Journal:  Ann Surg        ISSN: 0003-4932            Impact factor:   12.969


  17 in total

1.  Current concepts in pediatric burn care: the biology of cultured epithelial autografts: an eight-year study in pediatric burn patients.

Authors:  C C Compton
Journal:  Eur J Pediatr Surg       Date:  1992-08       Impact factor: 2.191

2.  Treatment of full skin thickness burn injury using cultured epithelial grafts.

Authors:  A Blight; E M Mountford; I M Cheshire; J M Clancy; P L Levick
Journal:  Burns       Date:  1991-12       Impact factor: 2.744

3.  Cultured epidermal autograft and the treatment of the massive burn injury.

Authors:  L R Haith; M L Patton; W T Goldman
Journal:  J Burn Care Rehabil       Date:  1992 Jan-Feb

4.  Addendum: multicenter experience with cultured epidermal autograft for treatment of burns.

Authors:  R Odessey
Journal:  J Burn Care Rehabil       Date:  1992 Jan-Feb

5.  Cultured epithelial autografts: three years of clinical experience with eighteen patients.

Authors:  P A Clugston; C F Snelling; I B Macdonald; H L Maledy; J C Boyle; E Germann; A D Courtemanche; P Wirtz; D J Fitzpatrick; D A Kester
Journal:  J Burn Care Rehabil       Date:  1991 Nov-Dec

6.  Grafting of burns with cultured epithelium prepared from autologous epidermal cells.

Authors: 
Journal:  Lancet       Date:  1981-01-10       Impact factor: 79.321

7.  [Use of keratinocyte cultures in treatment of severe burns--experiences up to now, outlook for further subsequent developments].

Authors:  G Henckel von Donnersmarck; W Mühlbauer; E Höfter; A Hartinger
Journal:  Unfallchirurg       Date:  1995-04       Impact factor: 1.000

8.  Cultured epidermal autografts: a life-saving and skin-saving technique in children.

Authors:  J J Coleman; B K Siwy
Journal:  J Pediatr Surg       Date:  1992-08       Impact factor: 2.545

9.  Lack of long-term durability of cultured keratinocyte burn-wound coverage: a case report.

Authors:  M H Desai; J M Mlakar; R L McCauley; K M Abdullah; R L Rutan; J P Waymack; M C Robson; D N Herndon
Journal:  J Burn Care Rehabil       Date:  1991 Nov-Dec

10.  Clinical trials of a living dermal tissue replacement placed beneath meshed, split-thickness skin grafts on excised burn wounds.

Authors:  J F Hansbrough; C Doré; W B Hansbrough
Journal:  J Burn Care Rehabil       Date:  1992 Sep-Oct
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  15 in total

1.  Skingineering I: engineering porcine dermo-epidermal skin analogues for autologous transplantation in a large animal model.

Authors:  Erik Braziulis; Thomas Biedermann; Fabienne Hartmann-Fritsch; Clemens Schiestl; Luca Pontiggia; Sophie Böttcher-Haberzeth; Ernst Reichmann; Martin Meuli
Journal:  Pediatr Surg Int       Date:  2011-03       Impact factor: 1.827

2.  Interleukin-1alpha and interleukin-6 enhance the antibacterial properties of cultured composite keratinocyte grafts.

Authors:  Gulsun Erdag; Jeffrey R Morgan
Journal:  Ann Surg       Date:  2002-01       Impact factor: 12.969

Review 3.  Hypertrophic scarring: the greatest unmet challenge after burn injury.

Authors:  Celeste C Finnerty; Marc G Jeschke; Ludwik K Branski; Juan P Barret; Peter Dziewulski; David N Herndon
Journal:  Lancet       Date:  2016-10-01       Impact factor: 79.321

Review 4.  Burns (Part 2). Tops and flops using cultured epithelial autografts in children.

Authors:  M Meuli; M Raghunath
Journal:  Pediatr Surg Int       Date:  1997-09       Impact factor: 1.827

5.  Use of porcine acellular dermal matrix as a dermal substitute in rats.

Authors:  A Srivastava; E Z DeSagun; L J Jennings; S Sethi; A Phuangsab; M Hanumadass; H M Reyes; R J Walter
Journal:  Ann Surg       Date:  2001-03       Impact factor: 12.969

6.  Cost-efficacy of cultured epidermal autografts in massive pediatric burns.

Authors:  J P Barret; S E Wolf; M H Desai; D N Herndon
Journal:  Ann Surg       Date:  2000-06       Impact factor: 12.969

7.  Optimizing in vitro culture conditions leads to a significantly shorter production time of human dermo-epidermal skin substitutes.

Authors:  Luca Pontiggia; Agnieszka Klar; Sophie Böttcher-Haberzeth; Thomas Biedermann; Martin Meuli; Ernst Reichmann
Journal:  Pediatr Surg Int       Date:  2013-02-03       Impact factor: 1.827

Review 8.  Stem Cells and Tissue Engineering: Regeneration of the Skin and Its Contents.

Authors:  Amy L Strong; Michael W Neumeister; Benjamin Levi
Journal:  Clin Plast Surg       Date:  2017-04-22       Impact factor: 2.017

9.  Functional characterization of cultured keratinocytes after acute cutaneous burn injury.

Authors:  Gerd G Gauglitz; Siegfried Zedler; Felix von Spiegel; Jasmin Fuhr; Guido Henkel von Donnersmarck; Eugen Faist
Journal:  PLoS One       Date:  2012-02-16       Impact factor: 3.240

10.  Human keratinocytes cultured on collagen matrix used as an experimental burn model.

Authors:  Christiane S Sobral; Alfredo Gragnani; Xudong Cao; Jeffrey R Morgan; Lydia Masako Ferreira
Journal:  J Burns Wounds       Date:  2007-10-30
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