| Literature DB >> 8812834 |
J J Calvete1, P F Varela, L Sanz, A Romero, K Mann, E Töpfer-Petersen.
Abstract
A method for the stepwise purification of bovine seminal plasma proteins aSFP, PDC-109, TIMP-2, RNAse, and BSP-30K/PDC-109 complex is presented. It is based on the use of nondenaturing, classical chromatographic procedures. The isolation protocol is reproducible, inexpensive, easy to carry out in a common laboratory, and yields large amounts of pure proteins for biochemical and structural studies.Entities:
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Year: 1996 PMID: 8812834 DOI: 10.1006/prep.1996.0073
Source DB: PubMed Journal: Protein Expr Purif ISSN: 1046-5928 Impact factor: 1.650