Literature DB >> 8812706

Site-Directed Mutagenesis in Epitope Mapping

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Abstract

Site-directed mutagenesis is a very useful tool for mapping and defining epitopes on protein antigens. This review discusses the methods used in, and the results of, studies on four different protein antigen systems. In addition, computer analyses and molecular modeling were used in an attempt to better understand the structural and energetic mechanisms underlying the effects observed following mutagenesis. The advantages and limitations of site-directed mutagenesis as an experimental tool are also discussed.

Year:  1996        PMID： 8812706     DOI： 10.1006/meth.1996.0058

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  27 in total

1.  Mapping the Energetic Epitope of an Antibody/Interleukin-23 Interaction with Hydrogen/Deuterium Exchange, Fast Photochemical Oxidation of Proteins Mass Spectrometry, and Alanine Shave Mutagenesis.

Authors:  Jing Li; Hui Wei; Stanley R Krystek; Derek Bond; Ty M Brender; Daniel Cohen; Jena Feiner; Nels Hamacher; Johanna Harshman; Richard Y-C Huang; Susan H Julien; Zheng Lin; Kristina Moore; Luciano Mueller; Claire Noriega; Preeti Sejwal; Paul Sheppard; Brenda Stevens; Guodong Chen; Adrienne A Tymiak; Michael L Gross; Lumelle A Schneeweis
Journal:  Anal Chem       Date:  2017-02-09       Impact factor: 6.986

2.  Delineating the functional map of the interaction between nimotuzumab and the epidermal growth factor receptor.

Authors:  Yaima Tundidor; Claudia Patricia García-Hernández; Amaury Pupo; Yanelys Cabrera Infante; Gertrudis Rojas
Journal:  MAbs       Date:  2014-04-23       Impact factor: 5.857

Review 3.  High throughput functional epitope mapping: revisiting phage display platform to scan target antigen surface.

Authors:  Gertrudis Rojas; Yaima Tundidor; Yanelys Cabrera Infante
Journal:  MAbs       Date:  2014       Impact factor: 5.857

4.  Determining Zebrafish Epitope Reactivity to Commercially Available Antibodies.

Authors:  Michael A Villarreal; Nicole M Biediger; Natalie A Bonner; Jennifer N Miller; Samantha K Zepeda; Benjamin J Ricard; Dana M García; Karen A Lewis
Journal:  Zebrafish       Date:  2017-03-20       Impact factor: 1.985

5.  A combinatorial mutagenesis approach for functional epitope mapping on phage-displayed target antigen: application to antibodies against epidermal growth factor.

Authors:  Yanelys Cabrera Infante; Amaury Pupo; Gertrudis Rojas
Journal:  MAbs       Date:  2014-03-03       Impact factor: 5.857

Review 6.  A high-throughput shotgun mutagenesis approach to mapping B-cell antibody epitopes.

Authors:  Edgar Davidson; Benjamin J Doranz
Journal:  Immunology       Date:  2014-09       Impact factor: 7.397

Review 7.  Biosensor-based epitope mapping of antibodies targeting the hemagglutinin and neuraminidase of influenza A virus.

Authors:  Zhu Guo; Jason R Wilson; Ian A York; James Stevens
Journal:  J Immunol Methods       Date:  2018-07-24       Impact factor: 2.303

8.  Topology characterization of a benzodiazepine-binding beta-rich domain of the GABAA receptor alpha1 subunit.

Authors:  Zhiwen Xu; Shisong Fang; Haifeng Shi; Hoiming Li; Yiqun Deng; Yinglei Liao; Jiun-Ming Wu; Hui Zheng; Huaimin Zhu; Hueih-Min Chen; Shui Ying Tsang; Hong Xue
Journal:  Protein Sci       Date:  2005-10       Impact factor: 6.725

9.  Antibody protection reveals extended epitopes on the human TSH receptor.

Authors:  Rauf Latif; Avelino Teixeira; Krzysztof Michalek; M Rejwan Ali; Max Schlesinger; Ramkumarie Baliram; Syed A Morshed; Terry F Davies
Journal:  PLoS One       Date:  2012-09-05       Impact factor: 3.240

10.  Identification and mapping of linear antibody epitopes in human serum albumin using high-density Peptide arrays.

Authors:  Lajla Bruntse Hansen; Soren Buus; Claus Schafer-Nielsen
Journal:  PLoS One       Date:  2013-07-23       Impact factor: 3.240
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