Continuous Cell Line from Pupal Ovary of Perina nuda (Lepidoptera: Lymantriidae) That Is Permissive to Nuclear Polyhedrosis Virus from P. nuda

A continuous cell line, designated NTU-PN-HH, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae). The cells have been through more than 300 passages during 4 years in TNM-FH medium supplemented with 10% FBS, at a constant temperature of 28°C. The cell line consists of four major morphologic types: polymorphic cells, spindle-shaped cells, round cells, and squamous cells. The characterization of this cell line showed that NTU-PN-HH is a newly established cell line. It is the first cell line that is permissive to PnNPV (P. nuda multiple nuclear polyhedrosis virus).