| Literature DB >> 8811450 |
A A Vitale1, C C Ferrari, H Aldana, J M Affanni.
Abstract
In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescence detection. This method is sensitive enough for detecting melatonin in a single pineal gland with a detection limit of 3 pg/mg tissue.Entities:
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Year: 1996 PMID： 8811450 DOI： 10.1016/0378-4347(96)00051-5
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495