Literature DB >> 8811105

One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins.

D Lafontaine1, D Tollervey.   

Abstract

With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.

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Year:  1996        PMID： 8811105      PMCID： PMC146098          DOI： 10.1093/nar/24.17.3469

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Improved method for high efficiency transformation of intact yeast cells.

Authors:  D Gietz; A St Jean; R A Woods; R H Schiestl
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

2.  The 18S rRNA dimethylase Dim1p is required for pre-ribosomal RNA processing in yeast.

Authors:  D Lafontaine; J Vandenhaute; D Tollervey
Journal:  Genes Dev       Date:  1995-10-15       Impact factor: 11.361

3.  A method for performing precise alterations in the yeast genome using a recycable selectable marker.

Authors:  F Längle-Rouault; E Jacobs
Journal:  Nucleic Acids Res       Date:  1995-08-11       Impact factor: 16.971

4.  A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.

Authors:  A Baudin; O Ozier-Kalogeropoulos; A Denouel; F Lacroute; C Cullin
Journal:  Nucleic Acids Res       Date:  1993-07-11       Impact factor: 16.971

5.  Deletion analysis of the Saccharomyces GAL gene cluster. Transcription from three promoters.

Authors:  T P St John; S Scherer; M W McDonell; R W Davis
Journal:  J Mol Biol       Date:  1981-10-25       Impact factor: 5.469

6.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Authors:  R S Sikorski; P Hieter
Journal:  Genetics       Date:  1989-05       Impact factor: 4.562

7.  SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs.

Authors:  M W Clark; M L Yip; J Campbell; J Abelson
Journal:  J Cell Biol       Date:  1990-11       Impact factor: 10.539

8.  A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.

Authors:  T Schimmang; D Tollervey; H Kern; R Frank; E C Hurt
Journal:  EMBO J       Date:  1989-12-20       Impact factor: 11.598
  8 in total
  58 in total

1.  Degradation of ribosomal RNA precursors by the exosome.

Authors:  C Allmang; P Mitchell; E Petfalski; D Tollervey
Journal:  Nucleic Acids Res       Date:  2000-04-15       Impact factor: 16.971

2.  An efficient recombination system for chromosome engineering in Escherichia coli.

Authors:  D Yu; H M Ellis; E C Lee; N A Jenkins; N G Copeland; D L Court
Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-23       Impact factor: 11.205

3.  Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability.

Authors:  D L Lafontaine; D Tollervey
Journal:  RNA       Date:  1999-03       Impact factor: 4.942

4.  DNA cloning using in vitro site-specific recombination.

Authors:  J L Hartley; G F Temple; M A Brasch
Journal:  Genome Res       Date:  2000-11       Impact factor: 9.043

5.  Functional characterization of nuclear localization signals in yeast Sm proteins.

Authors:  R Bordonné
Journal:  Mol Cell Biol       Date:  2000-11       Impact factor: 4.272

6.  Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3.

Authors:  A Colley; J D Beggs; D Tollervey; D L Lafontaine
Journal:  Mol Cell Biol       Date:  2000-10       Impact factor: 4.272

7.  Nob1p is required for cleavage of the 3' end of 18S rRNA.

Authors:  Alessandro Fatica; Marlene Oeffinger; Mensur Dlakić; David Tollervey
Journal:  Mol Cell Biol       Date:  2003-03       Impact factor: 4.272

8.  A complex pathway for 3' processing of the yeast U3 snoRNA.

Authors:  Joanna Kufel; Christine Allmang; Loredana Verdone; Jean Beggs; David Tollervey
Journal:  Nucleic Acids Res       Date:  2003-12-01       Impact factor: 16.971

9.  Nuclear pre-mRNA decapping and 5' degradation in yeast require the Lsm2-8p complex.

Authors:  Joanna Kufel; Cecile Bousquet-Antonelli; Jean D Beggs; David Tollervey
Journal:  Mol Cell Biol       Date:  2004-11       Impact factor: 4.272

10.  Rrp47p is an exosome-associated protein required for the 3' processing of stable RNAs.

Authors:  Philip Mitchell; Elisabeth Petfalski; Rym Houalla; Alexandre Podtelejnikov; Matthias Mann; David Tollervey
Journal:  Mol Cell Biol       Date:  2003-10       Impact factor: 4.272
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