Hydrolytic antibodies: variations on a theme.

Comparison of four independently-derived hydrolytic antibodies reveals striking similarities in their active sites. A common structural motif appears to be induced when the immune system is challenged with antigens containing aryl phosphonate and phosphonamidate groups, and key variations on this 'theme' must account for the observed differences in catalytic efficacy and mechanism. The limited structural repertoire accessed through standard immunization procedures suggests that new approaches may be needed to produce antibody catalysis with enzyme-like efficiencies.