CONCLUSION: Stimulation of afferent neurons by capsaicin exerts protective activity against cerulein-induced pancreatitis. This action is dependent on endogenous release of nitric oxide (NO). Deactivation of afferent neurons by high doses of capsaicin contributes to the severity of pancreatitis. This action involves mainly decreased pancreatic blood flow (PBF). Afferent nerves and NO cooperate in the maintenance of the integrity of pancreatic tissue. BACKGROUND: Stimulation of capsaicin-sensitive afferent fibers protects gastric mucosa against damage and causes changes in mucosal blood flow. The aim of the present study was to determine the role of stimulation or ablation of capsaicin-sensitive neurons and NO in the course of cerulein-induced pancreatitis in the rat. METHODS: Low and high doses of capsaicin were administered to animals with pancreatitis and to those without pancreatitis. The effect of several parameters was assessed. NO activity was blocked by NG-nitro-L-arginine. RESULTS: We found that a low dose of capsaicin administered intragastrically caused an increase in PBF. A neurotoxic dose of capsaicin caused a decrease in PBF, RNA content, and DNA synthesis. Pancreatitis led to a significant decrease in PBF and DNA synthesis, but an increase in pancreatic weight, protein content, plasma amylase concentration, and neutrophil adherence. Stimulatory doses of capsaicin attenuated the pancreatic tissue damage of pancreatitis, and alteration of PBF, DNA synthesis, and neutrophil adherence. Capsaicin-induced ablation of afferent neurons caused an increase in all indicators of pancreatic damage. Blocking NO enhanced pancreatic damage, and this was reversed by addition of L-arginine.
CONCLUSION: Stimulation of afferent neurons by capsaicin exerts protective activity against cerulein-induced pancreatitis. This action is dependent on endogenous release of nitric oxide (NO). Deactivation of afferent neurons by high doses of capsaicin contributes to the severity of pancreatitis. This action involves mainly decreased pancreatic blood flow (PBF). Afferent nerves and NO cooperate in the maintenance of the integrity of pancreatic tissue. BACKGROUND: Stimulation of capsaicin-sensitive afferent fibers protects gastric mucosa against damage and causes changes in mucosal blood flow. The aim of the present study was to determine the role of stimulation or ablation of capsaicin-sensitive neurons and NO in the course of cerulein-induced pancreatitis in the rat. METHODS: Low and high doses of capsaicin were administered to animals with pancreatitis and to those without pancreatitis. The effect of several parameters was assessed. NO activity was blocked by NG-nitro-L-arginine. RESULTS: We found that a low dose of capsaicin administered intragastrically caused an increase in PBF. A neurotoxic dose of capsaicin caused a decrease in PBF, RNA content, and DNA synthesis. Pancreatitis led to a significant decrease in PBF and DNA synthesis, but an increase in pancreatic weight, protein content, plasma amylase concentration, and neutrophil adherence. Stimulatory doses of capsaicin attenuated the pancreatic tissue damage of pancreatitis, and alteration of PBF, DNA synthesis, and neutrophil adherence. Capsaicin-induced ablation of afferent neurons caused an increase in all indicators of pancreatic damage. Blocking NO enhanced pancreatic damage, and this was reversed by addition of L-arginine.
Authors: Joanna Bonior; Piotr Ceranowicz; Ryszard Gajdosz; Beata Kuśnierz-Cabala; Piotr Pierzchalski; Zygmunt Warzecha; Artur Dembiński; Michał Pędziwiatr; Michalina Kot; Anna Leja-Szpak; Katarzyna Nawrot-Porąbka; Paweł Link-Lenczowski; Rafał Olszanecki; Krzysztof Bartuś; Jolanta Jaworek Journal: Int J Mol Sci Date: 2017-05-02 Impact factor: 5.923
Authors: Joanna Bonior; Zygmunt Warzecha; Piotr Ceranowicz; Ryszard Gajdosz; Piotr Pierzchalski; Michalina Kot; Anna Leja-Szpak; Katarzyna Nawrot-Porąbka; Paweł Link-Lenczowski; Michał Pędziwiatr; Rafał Olszanecki; Krzysztof Bartuś; Rafał Trąbka; Beata Kuśnierz-Cabala; Artur Dembiński; Jolanta Jaworek Journal: Int J Mol Sci Date: 2017-06-30 Impact factor: 5.923
Authors: Karolina Skonieczna-Żydecka; Ewa Stachowska; Dominika Maciejewska; Karina Ryterska; Joanna Palma; Maja Czerwińska-Rogowska; Mariusz Kaczmarczyk; Anna Gudan; Honorata Mruk; Barbara Świniarska; Justyna Kałduńska; Zofia Stachowska; Przemysław Mijal; Tomasz Mazur; Maciej Kupczyński; Wojciech Marlicz Journal: Int J Environ Res Public Health Date: 2018-10-15 Impact factor: 3.390