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Literature DB >> 8804835

Fluorescence studies of DNA and RNA structure and dynamics.

Abstract

Fluorescence spectroscopy is increasingly being used as a technique for probing the structure and dynamics of nucleic acids. Recently, fluorescence methods have been used to elucidate the three-dimensional arrangement of complex DNA and RNA structures, characterize structural perturbations resulting from base bulges in helices and junctions, determine helical handedness in solution, and analyze the protein-induced melting of DNA.

Mesh：

Substances：
DNA Probes
RNA
DNA

Year: 1996 PMID： 8804835 DOI： 10.1016/s0959-440x(96)80050-9

Source DB: PubMed Journal: Curr Opin Struct Biol ISSN： 0959-440X Impact factor: 6.809

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Cited

25 in total

1. Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes.

Authors: Irina Nazarenko; Rick Pires; Brian Lowe; Mohamad Obaidy; Ayoub Rashtchian
Journal: Nucleic Acids Res Date: 2002-05-01 Impact factor: 16.971

10. Characterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA binding, helical distortion, and cleavage compared with a canonical LAGLIDADG homing endonuclease.

Authors: Pawan Singh; Pankaj Tripathi; George H Silva; Alfred Pingoud; K Muniyappa
Journal: J Biol Chem Date: 2009-07-15 Impact factor: 5.157

Fluorescence studies of DNA and RNA structure and dynamics.

1. Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes.

2. Association of an RNA kissing complex analyzed using 2-aminopurine fluorescence.

3. Orientation and conformation of a lipase at an interface studied by molecular dynamics simulations.

4. Structural confirmation of a bent and open model for the initiation complex of T7 RNA polymerase.

5. Molecular recognition of HIV-1 RNAs with branched peptides.

6. Fluorescent pyrimidine ribonucleotide: synthesis, enzymatic incorporation, and utilization.

7. Pyrrolo-C as a fluorescent probe for monitoring RNA secondary structure formation.

8. Site-specific variations in RNA folding thermodynamics visualized by 2-aminopurine fluorescence.

9. Enzymatic incorporation of emissive pyrimidine ribonucleotides.

10. Characterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA binding, helical distortion, and cleavage compared with a canonical LAGLIDADG homing endonuclease.