Literature DB >> 8804431

Molecular cloning of rat phosphatidylinositol synthase cDNA by functional complementation of the yeast Saccharomyces cerevisiae pis mutation.

S Tanaka1, J Nikawa, H Imai, S Yamashita, K Hosaka.   

Abstract

Phosphatidylinositol synthase (CDP-1,2-diacyl-sn-glycerol: 3-phosphatidyltransferase, EC 2.7.8.11) catalyzes the formation of phosphatidylinositol and CMP from CDP-diacylglycerol and myo-inositol. We have cloned a phosphatidylinositol synthase cDNA from rat brain by functional complementation of the yeast pis mutation, which is defective in phosphatidylinositol synthase. The deduced protein comprised 213 amino acids with a calculated molecular mass of 23,613 Da. The predicted protein sequence is highly homologous to the previously determined yeast phosphatidylinositol synthase sequence. The cDNA hybridized to a 1.7-kb mRNA that was abundantly expressed in rat brain and kidney.

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Year:  1996        PMID： 8804431     DOI： 10.1016/0014-5793(96)00858-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  6 in total

1.  A highly dynamic ER-derived phosphatidylinositol-synthesizing organelle supplies phosphoinositides to cellular membranes.

Authors:  Yeun Ju Kim; Maria Luisa Guzman-Hernandez; Tamas Balla
Journal:  Dev Cell       Date:  2011-11-15       Impact factor: 12.270

2.  Cloning of Arabidopsis thaliana phosphatidylinositol synthase and functional expression in the yeast pis mutant.

Authors:  H W Xue; K Hosaka; G Plesch; B Mueller-Roeber
Journal:  Plant Mol Biol       Date:  2000-03       Impact factor: 4.076

3.  Resynthesis of phosphatidylinositol in permeabilized neutrophils following phospholipase Cbeta activation: transport of the intermediate, phosphatidic acid, from the plasma membrane to the endoplasmic reticulum for phosphatidylinositol resynthesis is not dependent on soluble lipid carriers or vesicular transport.

Authors:  J Whatmore; C Wiedemann; P Somerharju; P Swigart; S Cockcroft
Journal:  Biochem J       Date:  1999-07-15       Impact factor: 3.857

Review 4.  Phosphoinositides: tiny lipids with giant impact on cell regulation.

Authors:  Tamas Balla
Journal:  Physiol Rev       Date:  2013-07       Impact factor: 37.312

5.  Overexpression of phosphatidylinositol synthase enhances growth and G1 progression in NIH3T3 cells.

Authors:  Atsuko Deguchi; Kaoru Segawa; Kohei Hosaka; I Bernard Weinstein; Kazuo Umezawa
Journal:  Jpn J Cancer Res       Date:  2002-02

6.  Mapping the Substrate-Binding Sites in the Phosphatidylserine Synthase in Candida albicans.

Authors:  Yue Zhou; Chelsi D Cassilly; Todd B Reynolds
Journal:  Front Cell Infect Microbiol       Date:  2021-12-22       Impact factor: 5.293
  6 in total

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