Literature DB >> 880222

A rapid procedure for selectively isolating the major glycoprotein from purified rat brain myelin.

R H Quarles, C F Pasnak.   

Abstract

Treatment of the chloroform/methanol-insoluble residue of rat brain myelin with lithium 3,5-di-iodosalicylate solubilized the major myelin-associated glycoprotein along with most other proteins and glycoproteins. Equilibration of the extract with phenol resulted in the selective partitioning of the major glycoprotein into the aqueous phase, whereas nearly all of the other proteins and glycoproteins went into the phenol phase.

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Year:  1977        PMID: 880222      PMCID: PMC1164745          DOI: 10.1042/bj1630635

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  10 in total

1.  Ontogenetic study of a myelin-derived fraction with 2':3'-cyclic nucleotide 3'-phosphodydrolase activity higher than that of myelin.

Authors:  T V Waehneldt
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

2.  Variation of proteins, enzyme markers and gangliosides in myelin subfractions.

Authors:  J M Matthieu; R H Quarles; R O Brady; H de F Webster
Journal:  Biochim Biophys Acta       Date:  1973-12-05

3.  Characterization of the fraction obtained from the CNS of Jimpy mice by a procedure for myelin isolation.

Authors:  J M Matthieu; R H Quarles; H F de Webster; E L Hogan; R O Brady
Journal:  J Neurochem       Date:  1974-09       Impact factor: 5.372

4.  Myelination in rat brain: method of myelin isolation.

Authors:  W T Norton; S E Poduslo
Journal:  J Neurochem       Date:  1973-10       Impact factor: 5.372

5.  Evidence for the close association of a glycoprotein with myelin in rat brain.

Authors:  R H Quarles; J L Everly; R O Brady
Journal:  J Neurochem       Date:  1973-11       Impact factor: 5.372

6.  Partial characterization of a new myelin protein component.

Authors:  H C Agrawal; R M Burton; M A Fishman; R F Mitchell; A L Prensky
Journal:  J Neurochem       Date:  1972-09       Impact factor: 5.372

7.  Glycoproteins: isolation from cellmembranes with lithium diiodosalicylate.

Authors:  V T Marchesi; E P Andrews
Journal:  Science       Date:  1971-12-17       Impact factor: 47.728

8.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

9.  [35-S]sulfate incorporation into myelin glycoproteinsmi=entral nervous system.

Authors:  J M Matthieu; R H Quarles; J F Poduslo; R O Brady
Journal:  Biochim Biophys Acta       Date:  1975-05-05

10.  External labeling of galactose in surface membrane glycoproteins of the intact myelin sheath.

Authors:  J F Poduslo; R H Quarles; R O Brady
Journal:  J Biol Chem       Date:  1976-01-10       Impact factor: 5.157

  10 in total
  17 in total

1.  Heterogeneity of human anti-MAG IgM as revealed by their reactivity on avian embryonic tissues.

Authors:  G C Tucker; K Dellagi; C Schmitt; J C Brouet; J P Thiery
Journal:  Clin Exp Immunol       Date:  1987-02       Impact factor: 4.330

2.  Polyneuropathy associated with IgM monoclonal gammopathy. Immunological and pathological study in 31 patients.

Authors:  A Vital; C Vital; J Julien; A Baquey; A J Steck
Journal:  Acta Neuropathol       Date:  1989       Impact factor: 17.088

3.  Generation and characterization of mouse monoclonal antibodies to the myelin-associated glycoprotein (MAG).

Authors:  M J Dobersen; J A Hammer; A B Noronha; T D MacIntosh; B D Trapp; R O Brady; R H Quarles
Journal:  Neurochem Res       Date:  1985-04       Impact factor: 3.996

4.  Myelin-associated glycoprotein demonstrated immunocytochemically in myelin and myelin-forming cells of developing rat.

Authors:  N H Sternberger; R H Quarles; Y Itoyama; H D Webster
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

5.  Pattern of reactivity of IgM from the sera of eight patients with IgM monoclonal gammopathy and neuropathy with components of neural tissues: evidence for interaction with more than one epitope.

Authors:  F Lieberman; L S Marton; K Stefansson
Journal:  Acta Neuropathol       Date:  1985       Impact factor: 17.088

6.  Human monoclonal anti-MAG antibody and anti-Leu 7 recognise shared antigenic determinants in peripheral nerve and spinal cord.

Authors:  G Stoll; G Schwendemann; K Heininger; A J Steck; K V Toyka
Journal:  J Neurol Neurosurg Psychiatry       Date:  1985-07       Impact factor: 10.154

7.  Preparation and characterization of antisera to the myelin-associated glycoprotein.

Authors:  R H Quarles; D Johnson; R O Brady; N H Sternberger
Journal:  Neurochem Res       Date:  1981-10       Impact factor: 3.996

8.  IgM in a human neuropathy related to paraproteinemia binds to a carbohydrate determinant in the myelin-associated glycoprotein and to a ganglioside.

Authors:  A A Ilyas; R H Quarles; T D MacIntosh; M J Dobersen; B D Trapp; M C Dalakas; R O Brady
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

9.  Myelin-associated glycoprotein, a cell adhesion molecule of oligodendrocytes, is phosphorylated in brain.

Authors:  A M Edwards; M Arquint; P E Braun; J C Roder; R J Dunn; T Pawson; J C Bell
Journal:  Mol Cell Biol       Date:  1988-06       Impact factor: 4.272

10.  Molecular cloning and primary structure of myelin-associated glycoprotein.

Authors:  M Arquint; J Roder; L S Chia; J Down; D Wilkinson; H Bayley; P Braun; R Dunn
Journal:  Proc Natl Acad Sci U S A       Date:  1987-01       Impact factor: 11.205

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