Literature DB >> 8801224

Monoclonal antibody based competitive ELISA for the detection of antibodies against epizootic haemorrhagic disease of deer virus.

J A Thevasagayam1, T R Woolhouse, P P Mertens, J N Burroughs, J Anderson.   

Abstract

A monoclonal antibody based competitive enzyme-linked immunosorbent assay (MC-ELISA) for the detection of antibodies against epizootic haemorrhagic disease of deer viruses (EHDV) is described. Test sera were competed with a monoclonal antibody against the VP7 protein of EHDV serotype 1. The assay was capable of detecting antibodies to all serotypes of EHDV but unlike the agar gel immunodiffusion (AGID) test, gave no cross-reactions with antibodies against bluetongue, Palyam or Tilligery viruses. The MC-ELISA was more sensitive than a polyclonal based ELISA reported previously (Thevasagayam et al., 1995b) and would be ideal for epidemiological surveys since it is suitable for the examination of antisera from all animal species without the need for individual anti-species enzyme conjugates.

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Year:  1996        PMID： 8801224     DOI： 10.1016/0166-0934(95)01968-5

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  5 in total

1.  RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America.

Authors:  Narender S Maan; Sushila Maan; Kyriaki Nomikou; Donna J Johnson; Mehdi El Harrak; Hafsa Madani; Hagai Yadin; Serife Incoglu; Kadir Yesilbag; Andrew B Allison; David E Stallknecht; Carrie Batten; Simon J Anthony; Peter P C Mertens
Journal:  PLoS One       Date:  2010-09-17       Impact factor: 3.240

2.  Antigen capture competitive enzyme-linked immunosorbent assays using baculovirus-expressed antigens for diagnosis of bluetongue virus and epizootic hemorrhagic disease virus.

Authors:  J O Mecham; W C Wilson
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

3.  A serological survey of ruminant livestock in Kazakhstan during post-Soviet transitions in farming and disease control.

Authors:  M Lundervold; E J Milner-Gulland; C J O'Callaghan; C Hamblin; A Corteyn; A P Macmillan
Journal:  Acta Vet Scand       Date:  2004       Impact factor: 1.695

4.  Epizootic hemorrhagic disease in cattle, Western Turkey.

Authors:  Ethem Mutlu Temizel; Kadir Yesilbag; Carrie Batten; Sezgin Senturk; Narender S Maan; Peter Paul Clement Mertens; Hasan Batmaz
Journal:  Emerg Infect Dis       Date:  2009-02       Impact factor: 6.883

5.  Development of Real-Time RT-PCR Assays for Detection and Typing of Epizootic Haemorrhagic Disease Virus.

Authors:  N S Maan; S Maan; A C Potgieter; I M Wright; M Belaganahalli; P P C Mertens
Journal:  Transbound Emerg Dis       Date:  2016-02-17       Impact factor: 5.005
  5 in total

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