A novel method for purification of catalytic antibodies toward DNA from sera of patients with lymphoproliferative diseases.

A novel highly efficient method of purification of DNA-hydrolyzing antibodies from sera of patients with lymphoproliferative diseases was developed. The method suggests purification of serum sample using Cibacron Blue 3GA, ion exchange chromatography on DEAE-Sepharose FF, PrG affinity chromatography and selection of the DNA-binding fraction on DNA-Sephacryl. The application of the method allows to obtain the stable and active polyclonal preparation with increased content of DNA-hydrolyzing antibodies characterized by specific binding constant determined using heteropolymeric oligonucleotide substrate. The unusual properties of anti-DNA catalytic antibodies in respect to their interactions with DEAE ion exchanger are discussed.